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比较替米考星耐药和敏感副猪嗜血杆菌的转录组学分析。

Comparative transcriptional profiling of tildipirosin-resistant and sensitive Haemophilus parasuis.

机构信息

Veterinary Pharmacology Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P.R. China.

National Reference Laboratory of Veterinary Drug Residues and MAO Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agriculture University, Wuhan, 430070, P.R. China.

出版信息

Sci Rep. 2017 Aug 8;7(1):7517. doi: 10.1038/s41598-017-07972-5.

DOI:10.1038/s41598-017-07972-5
PMID:28790420
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5548900/
Abstract

Numerous studies have been conducted to examine the molecular mechanism of Haemophilus parasuis resistance to antibiotic, but rarely to tildipirosin. In the current study, transcriptional profiling was applied to analyse the variation in gene expression of JS0135 and tildipirosin-resistant JS32. The growth curves showed that JS32 had a higher growth rate but fewer bacteria than JS0135. The cell membranes of JS32 and a resistant clinical isolate (HB32) were observed to be smoother than those of JS0135. From the comparative gene expression profile 349 up- and 113 downregulated genes were observed, covering 37 GO and 63 KEGG pathways which are involved in biological processes (11), cellular components (17), molecular function (9), cellular processes (1), environmental information processing (4), genetic information processing (9) and metabolism (49) affected in JS32. In addition, the relative overexpression of genes of the metabolism pathway (HAPS_RS09315, HAPS_RS09320), ribosomes (HAPS_RS07815) and ABC transporters (HAPS_RS10945) was detected, particularly the metabolism pathway, and verified with RT-qPCR. Collectively, the gene expression profile in connection with tildipirosin resistance factors revealed unique and highly resistant determinants of H. parasuis to macrolides that warrant further attention due to the significant threat of bacterial resistance.

摘要

已经有许多研究致力于研究副猪嗜血杆菌对抗生素的耐药性的分子机制，但很少涉及替米考星。在本研究中，我们应用转录组学分析了 JS0135 和替米考星耐药的 JS32 之间基因表达的变化。生长曲线表明，JS32 的生长速度更高，但细菌数量少于 JS0135。观察到 JS32 和耐药临床分离株 (HB32) 的细胞膜比 JS0135 的更光滑。从比较基因表达谱中观察到 349 个上调基因和 113 个下调基因，涵盖了 37 个 GO 和 63 个 KEGG 途径，这些途径涉及生物过程 (11)、细胞成分 (17)、分子功能 (9)、细胞过程 (1)、环境信息处理 (4)、遗传信息处理 (9)和代谢 (49) 在 JS32 中受到影响。此外，还检测到代谢途径 (HAPS_RS09315、HAPS_RS09320)、核糖体 (HAPS_RS07815) 和 ABC 转运蛋白 (HAPS_RS10945) 的基因表达相对过表达，特别是代谢途径，并通过 RT-qPCR 进行了验证。总的来说，与替米考星耐药相关的基因表达谱揭示了副猪嗜血杆菌对大环内酯类药物的独特且高度耐药的决定因素，由于细菌耐药性的巨大威胁，值得进一步关注。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/f67b044b0a19/41598_2017_7972_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/ab2ad2952673/41598_2017_7972_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/af1e0b52ccfe/41598_2017_7972_Fig2_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/007f4d8906c1/41598_2017_7972_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/39c521ad563f/41598_2017_7972_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/fa262ded6e7c/41598_2017_7972_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/62a3924dd7f5/41598_2017_7972_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/f67b044b0a19/41598_2017_7972_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/ab2ad2952673/41598_2017_7972_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/af1e0b52ccfe/41598_2017_7972_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/ad59f2abe40c/41598_2017_7972_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/007f4d8906c1/41598_2017_7972_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/39c521ad563f/41598_2017_7972_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/fa262ded6e7c/41598_2017_7972_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/62a3924dd7f5/41598_2017_7972_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6424/5548900/f67b044b0a19/41598_2017_7972_Fig8_HTML.jpg

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