Sawada H, Tezuka H, Kirikae T, Yoshida Y, Uchino H, Mori K J
Leuk Res. 1986;10(12):1461-8. doi: 10.1016/0145-2126(86)90014-7.
Radiation-induced L8313 leukemia bearing mice (L8313 mice) had marked granulocytosis with splenomegaly. Hemopoietic stem cells and progenitors increased in the spleen but not in the bone marrow. Spleen conditioned-medium and serum from L8313 mice induced the formation of granulocyte-macrophage colonies (CFU-GM), erythroid bursts (BFU-E) and mixed colonies (CFU-Mix). Bone marrow conditioned medium did not show such activity. A cell line (STIL-3) was established from the spleen cells of L8313 mice. Surface marker analysis showed that the established cells were suppressor T cell. The cells produced IL-3 and GM-CSF in vitro, and induce essentially the same "leukemic" response in recipient mice. Inoculation of STIL-3 in diffusion chamber also induced leukemoid reaction, i.e. a marked granulocytosis with splenomegaly. Therefore, L8313 leukemia may be linked to an abnormality of growth and production of hemopoietic factors in hemopoietic regulatory cells.
辐射诱导的L8313白血病荷瘤小鼠（L8313小鼠）出现明显的粒细胞增多和脾肿大。造血干细胞和祖细胞在脾脏中增加，但在骨髓中未增加。L8313小鼠的脾脏条件培养基和血清可诱导粒细胞-巨噬细胞集落（CFU-GM）、红系爆式集落（BFU-E）和混合集落（CFU-Mix）的形成。骨髓条件培养基未显示出这种活性。从L8313小鼠的脾脏细胞中建立了一个细胞系（STIL-3）。表面标志物分析表明，所建立的细胞是抑制性T细胞。这些细胞在体外产生IL-3和GM-CSF，并在受体小鼠中诱导基本相同的“白血病”反应。将STIL-3接种到扩散室中也可诱导类白血病反应，即明显的粒细胞增多和脾肿大。因此，L8313白血病可能与造血调节细胞中造血因子的生长和产生异常有关。
