Sawada H, Itoh K, Kirikae T, Sakoda H, Tezuka H, Kuribayashi K, Maeda M, Yoshida Y, Uchino H, Hanaoka M
Department of Internal Medicine, Kokura Memorial Hospital, Kitakyushu, Japan.
Leuk Res. 1988;12(9):763-71. doi: 10.1016/0145-2126(88)90010-0.
Thy 1.2+ cells of L8313 leukemia bearing mice were previously shown to produce granulocyte macrophage colony stimulating activity (GM-CSA) and interleukin-3 (IL-3). Cell lines with the Thy 1.2+ phenotype were established from spleen cells of the leukemic mice, and were designated STIL-3 C5 and STIL-3 D10. They produced and released GM-CSA and IL-3 activities in culture supernatant. C5 cells were phenotypically Thy 1.2+ and Lyt 2.1+ with rearrangement of the T-cell receptor beta chain gene also being identified. D10 was Thy 1.2+ and L3T4+ with T-cell receptor beta chain gene rearrangement not detectable. Since the same sized rearranged bands were observed between C5 and L8313 leukemic mouse spleen cells, it is indicated that C5 cells are derived from the leukemic cells. Inoculation of these cells into mice induced granulocytosis. These results indicated that L8313, which was thought to be a "granulocytic leukemia", is actually a T-cell leukemia, whose granulocytosis is a leukemoid reaction induced by normal hemopoietic cells responding to the hemopoietic stimulating factors, GM-CSA and IL-3, produced by the leukemic T cells. Thus, L8313 should be known as a T-cell leukemia associated with granulocytosis.
先前已表明，携带L8313白血病的小鼠的Thy 1.2+细胞可产生粒细胞巨噬细胞集落刺激活性（GM-CSA）和白细胞介素-3（IL-3）。从白血病小鼠的脾细胞中建立了具有Thy 1.2+表型的细胞系，并将其命名为STIL-3 C5和STIL-3 D10。它们在培养上清液中产生并释放GM-CSA和IL-3活性。C5细胞在表型上为Thy 1.2+和Lyt 2.1+，同时也鉴定出T细胞受体β链基因发生了重排。D10为Thy 1.2+和L3T4+，未检测到T细胞受体β链基因重排。由于在C5和L8313白血病小鼠脾细胞之间观察到相同大小的重排条带，表明C5细胞来源于白血病细胞。将这些细胞接种到小鼠体内可诱导粒细胞增多。这些结果表明，L8313原本被认为是一种“粒细胞白血病”，实际上是一种T细胞白血病，其粒细胞增多是正常造血细胞对白血病T细胞产生的造血刺激因子GM-CSA和IL-3作出反应而引发的类白血病反应。因此，L8313应被称为与粒细胞增多相关的T细胞白血病。
