Rowley H L, Martin K F, Marsden C A
Department of Physiology and Pharmacology, University of Nottingham Medical School, UK.
J Neurosci Methods. 1995 Mar;57(1):93-9. doi: 10.1016/0165-0270(94)00132-z.
The measurement of amino acid neurotransmitters by high-performance liquid chromatography (HPLC) has emerged as a reliable and sensitive method. This paper describes a method which employs electrochemical (EC) detection of amino acid derivatives formed by a reaction with o-phthalaldehyde (OPA) in the presence of sulphite ions. This is discussed in relation to the problems of previously reported methods based on OPA derivatisation. Precise separation of the following 7 amino acid standards is achieved using isocratic elution: serine, glycine, taurine, glutamate, arginine, alanine and GABA, in order of increasing retention time. Total elution time is 25 min. Derivatisation proceeds at room temperature and the derivatives are stable for up to 5 h. This technique has the sensitivity to determine the concentrations of amino acid neurotransmitters in cerebrospinal fluid (CSF) and an in vivo microdialysis method is discussed for the detection of basal and potassium-stimulated levels of gamma-aminobutyric acid and glutamate from rat hippocampus.
采用高效液相色谱法（HPLC）测定氨基酸神经递质已成为一种可靠且灵敏的方法。本文描述了一种方法，该方法利用电化学（EC）检测在亚硫酸根离子存在下与邻苯二甲醛（OPA）反应形成的氨基酸衍生物。本文结合先前报道的基于OPA衍生化方法的问题对此进行了讨论。使用等度洗脱可实现以下7种氨基酸标准品的精确分离：丝氨酸、甘氨酸、牛磺酸、谷氨酸、精氨酸、丙氨酸和γ-氨基丁酸（GABA），按保留时间增加的顺序排列。总洗脱时间为25分钟。衍生化在室温下进行，衍生物在长达5小时内稳定。该技术具有测定脑脊液（CSF）中氨基酸神经递质浓度的灵敏度，并讨论了一种用于检测大鼠海马体中γ-氨基丁酸和谷氨酸基础水平及钾刺激水平的体内微透析方法。
