水叶草科的新型微卫星开发与表征
Novel microsatellite development and characterization for (Hydrophyllaceae).
作者信息
Riser James P, Schwabe Anna L, Neale Jennifer Ramp
机构信息
Denver Botanic Gardens, 909 York Street, Denver, Colorado 80206 USA.
出版信息
Appl Plant Sci. 2017 Jul 19;5(7). doi: 10.3732/apps.1700030. eCollection 2017 Jul.
PREMISE OF THE STUDY
Microsatellite primers were developed to characterize genetic diversity and structuring in the genus (Hydrophyllaceae) and to further conservation efforts for .
METHODS AND RESULTS
Fifteen novel microsatellite primers were developed for . These were characterized for genetic variation in three separate populations. Two to nine alleles were found per locus. Overall observed heterozygosity and expected heterozygosity ranged from 0.000 to 0.800 and 0.000 to 0.840, respectively. Additionally, these loci were successfully amplified and showed polymorphism in and a potential new species.
CONCLUSIONS
These microsatellite markers will be useful in assessing genetic diversity, structuring, and gene flow within and among populations of the rare , in addition to related species. These markers will provide important genetic data needed for appropriate conservation and management of these rare plants.
研究前提
开发微卫星引物以表征水叶草科某属的遗传多样性和结构，并进一步推动该属的保护工作。
方法与结果
为该属开发了15种新型微卫星引物。在三个不同的该属种群中对其遗传变异进行了表征。每个位点发现2至9个等位基因。总体观察杂合度和预期杂合度分别为0.000至0.800和0.000至0.840。此外，这些位点在该属及一个潜在的新该属物种中成功扩增并表现出多态性。
结论
这些微卫星标记将有助于评估珍稀该属种群内部和种群之间的遗传多样性、结构和基因流动，以及相关的该属物种。这些标记将为这些珍稀植物的适当保护和管理提供所需的重要遗传数据。
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