González-Robles Ana, Manzaneda Antonio J, Bastida Jesús M, Harvey Nick, Jaime Rafael, Salido Teresa, Martínez Luisa M, Fernández-Ocaña Ana, Alcántara Julio M, Rey Pedro J
Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, E-23071 Jaén, Spain.
Genetic Marker Services, Brighton, United Kingdom.
Appl Plant Sci. 2016 Dec 8;4(12). doi: 10.3732/apps.1600092. eCollection 2016 Dec.
Microsatellite primers were developed to characterize and evaluate patterns of genetic diversity and structure in the endangered Mediterranean shrub (Rhamnaceae).
Twenty microsatellite primers were developed for , of which 14 were polymorphic. We evaluated microsatellite polymorphism in 97 specimens from 18 Spanish and seven Moroccan populations. Between two and eight alleles were found per locus, and the average number of alleles was 5.54. Observed heterozygosity and expected heterozygosity ranged from 0.08 to 0.90 and from 0.08 to 0.82, respectively. Nine of these primers also amplified microsatellite loci in .
The microsatellite markers described here will be useful in studies on genetic variation, population genetic structure, and gene flow in the fragmented habitat of this species. These markers are a valuable resource for designing appropriate conservation measures for the species in the Mediterranean range.
开发微卫星引物以表征和评估濒危地中海灌木（鼠李科）的遗传多样性和结构模式。
为[物种名称]开发了20个微卫星引物，其中14个具有多态性。我们评估了来自18个西班牙和7个摩洛哥种群的97个样本中的微卫星多态性。每个位点发现2至8个等位基因，等位基因的平均数量为5.54。观察到的杂合度和预期杂合度分别为0.08至0.90和0.08至0.82。其中9个引物也在[另一物种名称]中扩增出微卫星位点。
这里描述的微卫星标记将有助于研究该物种破碎化栖息地中的遗传变异、种群遗传结构和基因流动。这些标记是为地中海地区该物种设计适当保护措施的宝贵资源。
