Department of Head and Neck Radiotherapy, The First People's Hospital of Foshan, Foshan, Guangdong 528000, P.R. China.
Mol Med Rep. 2017 Oct;16(4):4887-4894. doi: 10.3892/mmr.2017.7163. Epub 2017 Aug 3.
Breast cancer is one of the most frequently occurring malignancies in female cancers worldwide, however, its detailed mechanism of tumorigenesis remains to be elucidated. Long non-coding RNAs (LncRNAs) have previously been demonstrated to be important in multiple cancers, including breast cancer. The present study aimed to elucidate the molecular mechanism of the effects of the novel Lnc RNA HOXA11‑AS, on cell proliferation and metastasis in breast cancer. The data revealed that the relative transcript level of HOXA11‑AS was upregulated in vivo and in vitro in models of breast cancer. Knockdown of HOXA11‑AS in MDA‑MB‑231 and MDA‑MB‑436 breast cancer cell lines inhibited the formation of cell colonies and arrested the cell cycle at the G0/G1 phase. Depletion of HOXA11‑AS using two specific short interfering (si)RNAs against HOXA11‑AS (siHOXA11‑AS‑1 and siHOXA11‑AS‑2) additionally suppressed the cell proliferative rate. Furthermore, transwell assays and wound‑healing analysis revealed that siRNA transfection inhibited cell migration and invasion by ~50% in the two cell lines. The results of the present study demonstrated the oncogenic role of HOXA11‑AS in breast cancer, providing novel clues for the future clinical diagnosis and treatment of early stage breast cancer patients.
乳腺癌是全球女性癌症中最常见的恶性肿瘤之一，但肿瘤发生的详细机制仍有待阐明。长链非编码 RNA（lncRNA）已被证明在多种癌症中具有重要作用，包括乳腺癌。本研究旨在阐明新型 LncRNA HOXA11-AS 对乳腺癌细胞增殖和转移的影响的分子机制。数据显示，HOXA11-AS 的相对转录水平在乳腺癌的体内和体外模型中均上调。在 MDA-MB-231 和 MDA-MB-436 乳腺癌细胞系中敲低 HOXA11-AS 抑制细胞集落的形成，并将细胞周期阻滞在 G0/G1 期。用两种针对 HOXA11-AS 的特异性短发夹 RNA（siRNA）（siHOXA11-AS-1 和 siHOXA11-AS-2）耗尽 HOXA11-AS 还抑制了细胞增殖率。此外，Transwell 分析和划痕愈合分析显示，siRNA 转染抑制了两种细胞系中约 50%的细胞迁移和侵袭。本研究结果表明 HOXA11-AS 在乳腺癌中具有致癌作用，为未来早期乳腺癌患者的临床诊断和治疗提供了新的线索。
