通过伤口愈合试验分析表皮生长因子受体诱导的细胞运动性

Analysis of Epidermal Growth Factor Receptor-Induced Cell Motility by Wound Healing Assay.

作者信息

Tong Junfeng, Wang Zhixiang

机构信息

Signal Transduction Research Group, Department of Medical Genetics, Faculty of Medicine and Dentistry, University of Alberta, 835 MSB, 114 St. NW, Edmonton, AB, Canada, T6G 2H7.

出版信息

Methods Mol Biol. 2017;1652:159-163. doi: 10.1007/978-1-4939-7219-7_12.

DOI:10.1007/978-1-4939-7219-7_12

PMID:28791642

Abstract

Wound healing assays are well-defined and low-cost assays to study cell proliferation and migration rates of different cells and culture conditions as well as cell polarity, tissue matrix remodeling, and actin cytoskeletal structure regulation. The assay procedure generally involves growing a confluent cell monolayer and then creating a wound by scratching a line through the monolayer to destroy or displace certain cells. The open gap created by this wound is healed as cells move in and fill the damaged area. This wound healing process can take several hours to days depending on the cell type, culture conditions, and the width of the wound. The healing process is investigated microscopically over certain time intervals as the cells move into the open gap and close the wound.

摘要

伤口愈合分析是明确且低成本的分析方法，用于研究不同细胞和培养条件下的细胞增殖与迁移速率，以及细胞极性、组织基质重塑和肌动蛋白细胞骨架结构调控。该分析过程通常包括培养汇合的细胞单层，然后通过在单层上划一条线来制造伤口，以破坏或取代某些细胞。这个伤口产生的开放间隙会随着细胞移入并填充受损区域而愈合。根据细胞类型、培养条件和伤口宽度，这个伤口愈合过程可能需要数小时到数天。在细胞移入开放间隙并闭合伤口的特定时间间隔内，通过显微镜观察来研究愈合过程。