Freitas Juliano T, Jozic Ivan, Bedogni Barbara
Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, FL, USA.
Methods Mol Biol. 2021;2265:65-71. doi: 10.1007/978-1-0716-1205-7_4.
Cell migration is a critical process involved in morphogenesis, inflammation, and cancer metastasis. Wound healing assay is a simple, non-expensive, and highly reproducible method to study cancer cell migration in vitro. It is based on the observation that cells growing in a monolayer migrate to re-establish cell contacts after the development of an artificial wound. The assay involves creation of a wound in a monolayer, image acquisition during wound closure, and comparison of migrated area at initial and final time points.
细胞迁移是一个涉及形态发生、炎症和癌症转移的关键过程。伤口愈合实验是一种简单、经济且高度可重复的体外研究癌细胞迁移的方法。它基于这样的观察：在形成人工伤口后，单层生长的细胞会迁移以重新建立细胞接触。该实验包括在单层中制造伤口、在伤口闭合过程中采集图像，以及比较初始和最终时间点的迁移面积。
