黑色素瘤细胞迁移的伤口愈合试验

Wound Healing Assay for Melanoma Cell Migration.

作者信息

Freitas Juliano T, Jozic Ivan, Bedogni Barbara

机构信息

Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, FL, USA.

出版信息

Methods Mol Biol. 2021;2265:65-71. doi: 10.1007/978-1-0716-1205-7_4.

DOI:10.1007/978-1-0716-1205-7_4

PMID:33704705

Abstract

Cell migration is a critical process involved in morphogenesis, inflammation, and cancer metastasis. Wound healing assay is a simple, non-expensive, and highly reproducible method to study cancer cell migration in vitro. It is based on the observation that cells growing in a monolayer migrate to re-establish cell contacts after the development of an artificial wound. The assay involves creation of a wound in a monolayer, image acquisition during wound closure, and comparison of migrated area at initial and final time points.

摘要

细胞迁移是一个涉及形态发生、炎症和癌症转移的关键过程。伤口愈合实验是一种简单、经济且高度可重复的体外研究癌细胞迁移的方法。它基于这样的观察：在形成人工伤口后，单层生长的细胞会迁移以重新建立细胞接触。该实验包括在单层中制造伤口、在伤口闭合过程中采集图像，以及比较初始和最终时间点的迁移面积。