School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529020, People's Republic of China.
Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou, 510640, People's Republic of China.
Mol Biol Rep. 2020 Jan;47(1):507-519. doi: 10.1007/s11033-019-05157-z. Epub 2019 Oct 30.
Liver cancer, one of the most common malignancies, is the second leading cause of cancer death in the world. The citrus reticulate peel and black tea have been studied for their beneficial health effects. In spite of the many studies have been reported, the underlying molecular mechanisms underlying its health benefits are still not fully understood. In present study, we developed a unique citrus reticulate peel black tea (CRPBT) by combined citrus reticulate peel and black tea and assessed its active ingredients, anti-oxidant and anti-liver cancer effects in vitro. The results suggested that CRPBT exhibited antioxidant capacity and effectively inhibited proliferation and migration of liver cancer cells in a dose- and time- dependent manner. Mechanistically, CRPBT significantly down-regulated phosphorylation of PI3K and AKT, and up-regulated the ratio of Bax/Bcl-2, and suppressed the expression of MMP2/9, N-cadherin and Vimetin proteins in liver cancer cells. Taken together, CRPBT has good effect on inhibiting migration, invasion, proliferation, and inducing apoptosis in liver cancer cells.
肝癌是最常见的恶性肿瘤之一，是世界上癌症死亡的第二大主要原因。柑橘皮和红茶已被研究其有益健康的作用。尽管已经有很多研究报告，但它对健康的潜在分子机制仍不完全清楚。在本研究中，我们通过将柑橘皮和红茶相结合开发了一种独特的柑橘皮红茶（CRPBT），并评估了其在体外的活性成分、抗氧化和抗肝癌作用。结果表明，CRPBT 表现出抗氧化能力，并能有效抑制肝癌细胞的增殖和迁移，呈剂量和时间依赖性。在机制上，CRPBT 显著下调了 PI3K 和 AKT 的磷酸化，并上调了 Bax/Bcl-2 的比值，抑制了肝癌细胞中 MMP2/9、N-钙黏蛋白和波形蛋白的表达。综上所述，CRPBT 对抑制肝癌细胞的迁移、侵袭、增殖和诱导凋亡有很好的效果。
