Liu Zhen-Shu, Chen Po-Wen, Wang Jung-Yu, Kuo Tai-Chen
1 Department of Safety, Health and Environmental Engineering, Ming Chi University of Technology, New Taipei City, Taiwan, Republic of China.
2 Department of Nursing, St. Mary's Junior College of Medicine, Nursing and Management, Yilan 26644, Taiwan, Republic of China (ORCID: http://orcid.org/0000-0003-1230-1532 ).
J Food Prot. 2017 Sep;80(9):1489-1495. doi: 10.4315/0362-028X.JFP-17-052.
Coffee is a popular beverage worldwide, but coffee beans can be contaminated with carcinogens. The Ames Salmonella mutagenicity test is often used for analysis of carcinogens for mutagenicity. However, previous studies have provided controversial data about the direct mutagenicity of coffee beans based on Ames test results. This study was conducted to determine the mutagenicity of popular Americano coffee based on results from the Ames test. Coffee samples without additives that were served by five international coffee chain restaurants were subjected to the analysis using Salmonella Typhimurium tester strains TA98, TA100, and TA1535. The levels of bacterial revertants in samples from coffee chains were lower than the twofold criterion of the control sets, and no significant dose-response effect was observed with or without rat liver enzyme activation. These data indicate that Americano coffees from the selected coffee chains possessed no direct mutagenic activity with or without enzyme activation. These findings suggest a low mutagenic risk from Americano coffees served by the selected coffee chains and support the use of other methods to confirm the nonmutagenicity of coffee products. These results are consistent with most recent epidemiological reports.
咖啡是全球广受欢迎的饮品，但咖啡豆可能被致癌物污染。艾姆斯沙门氏菌致突变性试验常用于分析致癌物的致突变性。然而，以往的研究基于艾姆斯试验结果，给出了关于咖啡豆直接致突变性的有争议数据。本研究旨在根据艾姆斯试验结果确定流行的美式咖啡的致突变性。对五家国际咖啡连锁店提供的无添加剂咖啡样品，使用鼠伤寒沙门氏菌测试菌株TA98、TA100和TA1535进行分析。咖啡连锁店样品中的细菌回复突变体水平低于对照组两倍的标准，无论有无大鼠肝酶激活，均未观察到显著的剂量反应效应。这些数据表明，所选咖啡连锁店的美式咖啡无论有无酶激活均无直接致突变活性。这些发现表明，所选咖啡连锁店提供的美式咖啡致突变风险较低，并支持使用其他方法来确认咖啡产品的非致突变性。这些结果与最近的流行病学报告一致。
