Albaladejo Vicente R, Villanueva Orbaiz R, Ortega Molina P, Astasio Arbiza P, Gil Miguel A, Granados Arroyo B, Calle Puron M E, Domínguez Rojas V
Cátedra de Medicina Preventiva y Salud Pública, Universidad Complutense de Madrid, España.
Rev Esp Salud Publica. 1995 Sep-Oct;69(5):393-408.
The sources of potential mutagens in our environment are many, but the most important of these is water for public consumption. This is a result of the chlorinating process which is the main reason for the appearance of these mutagens. With this in mind, the aim of our study was to check a possible mutagenic activity, using the Ames test, in organic concentrates taken from water for public consumption in Madrid.
Several bacterial strains were used, namely Salmonella histidine dependent TA1535, TA1538, TA98 and TA100, taken originally from Salmonella typhimurium LT2. Each test was performed twice, with or without the introduction of the mammalian-microsome activation (S9 mix), as per the indications in Ames. The plate incorporation assay was used to test the mutagenicity. All samples of the water in question were processed and treated so as to create concentrates of organic chlorinated compounds.
The highest levels of mutagenicity appeared in the TA1535 strain and in the tests where the microsome fraction was not used (IM = 1.94).
With regard to mutagenic evaluation in organic concentrates taken from water for public consumption, no positive activity was found in any of the tester strains.
我们环境中潜在诱变剂的来源众多，但其中最重要的是公共饮用水。这是氯化过程的结果，而氯化过程是这些诱变剂出现的主要原因。考虑到这一点，我们研究的目的是使用艾姆斯试验检测从马德里公共饮用水中提取的有机浓缩物中可能存在的诱变活性。
使用了几种细菌菌株，即最初源自鼠伤寒沙门氏菌LT2的组氨酸依赖型沙门氏菌TA1535、TA1538、TA98和TA100。根据艾姆斯试验的说明，每次试验均进行两次，分别在有或没有引入哺乳动物微粒体激活剂（S9混合物）的情况下进行。采用平板掺入法检测诱变性。对所有相关水样进行处理，以制备有机氯化合物浓缩物。
最高诱变水平出现在TA1535菌株以及未使用微粒体部分的试验中（诱变指数=1.94）。
关于从公共饮用水中提取的有机浓缩物的诱变评估，在任何测试菌株中均未发现阳性活性。
