Noal Charlise Bolson, Monteiro Danieli Urach, Brum Thiele Faccim de, Emmanouilidis Jessica, Zanette Regis Adriel, Morel Ademir Farias, Stefanon Eliza Beti de Cassia, Frosi Marina, la Rue Mario Luiz de
Universidade Federal de Santa Maria. Departamento de Microbiologia e Parasitologia, Santa Maria, Rio Grande do Sul, Brazil.
Universidade Federal de Santa Maria, Departamento de Farmácia Industrial, Santa Maria, Rio Grande do Sul, Brazil.
Rev Inst Med Trop Sao Paulo. 2017;59:e42. doi: 10.1590/S1678-9946201759042. Epub 2017 Aug 3.
Scolicidal agents are important in the treatment of cystic echinococcosis. This study evaluated the scolicidal activity of the plant Blepharocalyx salicifolius (H.B.K.) Berg against Echinococcus ortleppi protoscoleces. The parasite species was identified by amplifying a fragment of the gene cytochrome c oxidase subunit 1 (COX 1). B. salicifolius crude extract at concentrations of 100, 200, 300 and 400 mg/mL was analyzed at different times (5, 10, 15, 30, 45 and 60 min). N-butanol and ethyl acetate fractions (100 and 200 mg/ mL) were also analyzed at 5, 10, 15 and 30 min. Both fractions showed 100% scolicidal activity at the concentration of 200 mg/mL at 5 min. Gallic acid, identified as the major compound of the ethyl acetate fraction- was responsible for the observed scolicidal activity. The results showed that crude extract and fractions of B. salicifolius have scolicidal effect against E. ortleppi protoscoleces.
杀头节片剂在囊性棘球蚴病的治疗中很重要。本研究评估了植物柳叶帽蕊木（Blepharocalyx salicifolius (H.B.K.) Berg）对奥氏棘球绦虫原头节的杀头节片活性。通过扩增细胞色素c氧化酶亚基1（COX 1）基因片段来鉴定寄生虫种类。在不同时间点（5、10、15、30、45和60分钟）分析浓度为100、200、300和400 mg/mL的柳叶帽蕊木粗提物。还在5、10、15和30分钟时分析了正丁醇和乙酸乙酯馏分（100和200 mg/mL）。两种馏分在5分钟时浓度为200 mg/mL时均显示出100%的杀头节片活性。被鉴定为乙酸乙酯馏分主要化合物的没食子酸是观察到的杀头节片活性的原因。结果表明，柳叶帽蕊木的粗提物和馏分对奥氏棘球绦虫原头节有杀头节片作用。
