Mahmoudvand Hossein, Ezatpour Behrouz, Rashidipour Marzieh, Mirbadie Seyed Reza, Mahmoudvand Hormoz
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Young Researchers and Elite Club, Islamic Azad University, Khorramabad Branch, Khorramabad, Iran.
Pak J Pharm Sci. 2016 Nov;29(6):2125-2128.
Current scolicidal agents, which have been used for inactivation of protoscoleces during hydatid cyst surgery are associated with adverse side effects. This study aims to evaluate the in vitro scolicidal effects of Nectaroscordum tripedale L. leave extract against protoscoleces of hydatid cysts and its acute toxicity in mice model. Various concentrations of the extract (12.5-100 mg/mL) were used for 5 to 30 min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). In addition, the acute toxicity of N. tripedale extract was determined for 2 days in mice model. The results showed that the N. tripedale extract at the concentration of 100 mg/mL after 5 min of exposure killed 100% protoscoleces. Similarly, the mean of mortality rate of protoscoleces after 10 min of exposure to concentration of 50 mg/mL was 100%. The LD values of intraperitoneal injection of the N. tripedale extract was 3.36 g/kg body wt. and the maximum nonfatal doses were 2.98 g/kg body wt. The results showed the potential of N. tripedale extract as a natural source for the production of new scolicidal agent for use in hydatid cyst surgery.
目前用于包虫囊肿手术中原头蚴灭活的杀头节剂存在不良副作用。本研究旨在评估紫花地丁叶提取物对包虫囊肿原头蚴的体外杀头节作用及其在小鼠模型中的急性毒性。使用不同浓度的提取物（12.5 - 100 mg/mL）作用5至30分钟。使用伊红排斥试验（0.1%伊红染色）确认原头蚴的活力。此外，在小鼠模型中测定了紫花地丁提取物的急性毒性，为期2天。结果表明，浓度为100 mg/mL的紫花地丁提取物作用5分钟后可杀死100%的原头蚴。同样，暴露于50 mg/mL浓度1分钟后原头蚴的平均死亡率为100%。腹腔注射紫花地丁提取物的LD值为3.36 g/kg体重，最大非致死剂量为2.98 g/kg体重。结果表明，紫花地丁提取物有潜力作为生产用于包虫囊肿手术的新型杀头节剂的天然来源。
