微小RNA-454可能通过靶向AKT在三阴性乳腺癌中发挥癌基因的作用。

MicroRNA-454 may function as an oncogene via targeting AKT in triple negative breast cancer.

作者信息

Li Qun, Liu Jia, Meng Xianying, Pang Renzhu, Li Jie

机构信息

The First Hospital, Jilin University, Changchun, Jilin China.

出版信息

J Biol Res (Thessalon). 2017 Aug 3;24:10. doi: 10.1186/s40709-017-0067-x. eCollection 2017 Dec.

BACKGROUND

Altered microRNAs expression mediates tumor development and progression in many type cancers including triple negative breast cancer (TNBC). Here we detected the effect of miR-454 on cell proliferation, migration and invasion of triple negative breast cancer cells.

RESULTS

miR-454 promoted the proliferation of TNBC, and enhanced migration and invasion in TNBC cells. Meanwhile, miR-454 improved the survival of TNBC cells after ironizing radiation. miR-454 inhibited radiation-induced apoptosis in TNBC cells by regulation of caspase 3/7 and Bcl-2 expression. Furthermore, PTEN and pAKT levels in TNBC cells were changed after overexpression of miR-454.

CONCLUSIONS

miR-454 played an essential role in tumor development and progression in TNBC, and might be used as a potential biomarker to predict radiotherapy response and prognosis in TNBC.

背景

微小RNA表达的改变介导了包括三阴性乳腺癌（TNBC）在内的多种类型癌症的肿瘤发生和进展。在此，我们检测了miR-454对三阴性乳腺癌细胞增殖、迁移和侵袭的影响。

结果

miR-454促进了TNBC的增殖，并增强了TNBC细胞的迁移和侵袭能力。同时，miR-454提高了TNBC细胞在电离辐射后的存活率。miR-454通过调节半胱天冬酶3/7和Bcl-2的表达抑制TNBC细胞中辐射诱导的凋亡。此外，miR-454过表达后，TNBC细胞中的PTEN和pAKT水平发生了变化。