Lepper A W, Moore L J, Atwell J L, Tennent J M
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Vic., Australia.
Vet Microbiol. 1992 Sep;32(2):177-87. doi: 10.1016/0378-1135(92)90104-2.
Three groups of ten calves were each immunised with a total of 400 micrograms pili prepared from three separate strains of Moraxella bovis in Alhydrogel-oil adjuvant as two divided, equal doses 21 days apart. Groups 1 and 2 each received a monovalent vaccine made from strain 4L and S276R respectively, which belonged to pili serogroup A. Group 3 received vaccine made from pili of strain Maff1, belonging to serogroup F. A further group of ten calves served as non-vaccinated controls. Calves in groups 1 and 2 had developed serogroup A-specific antibody and those in group 3 developed serogroup F-specific antibody, and some evidence of cross-reacting antibody was also detected when measured by an agglutination test using formalin-killed piliated cells of serogroup A strain 4L. Although antibody titres measured against purified pili by ELISA were highest with homologous serogroup antigens, cross-reactive titres to shared epitopes of M. bovis pili were also detected by this method. Ocular challenge of the 40 calves with virulent M. bovis of serogroup A strain S276R was carried out 14 days after the second vaccine dose. All non-vaccinated calves developed infectious bovine keratoconjunctivitis (IBK). The percentage protection in groups 1 (strain 4L) and 2 (strain S276R) was 60% and 80% respectively (P less than 0.05), with mean lesion scores of 0.7 and 0.3 out of a possible 6.0. The percentage protection of calves in group 3 (strain Maff1) was only 30%, with a mean lesion score of 1.4 compared with 2.2 for non-vaccinated controls. The present findings, together with other evidence indicating that immunity to IBK is serogroup-specific, suggest that inclusion of pili from one representative strain from each of the seven Australian and British serogroups in a polyvalent, subunit vaccine should effectively protect the majority of cattle against IBK caused by most field strains of M. bovis encountered in Australia and the United Kingdom.
将三组每组十头小牛，分别用从三株不同的牛莫拉菌菌株制备的共400微克菌毛，在铝胶油佐剂中以两个分开的、等量的剂量免疫，间隔21天。第1组和第2组分别接受由4L株和S276R株制成的单价疫苗，这两株属于菌毛血清群A。第3组接受由Maff1株菌毛制成的疫苗，属于血清群F。另外一组十头小牛作为未接种疫苗的对照。第1组和第2组的小牛产生了血清群A特异性抗体，第3组的小牛产生了血清群F特异性抗体，并且当用血清群A菌株4L的福尔马林灭活菌毛细胞进行凝集试验测量时，也检测到了一些交叉反应抗体的证据。在第二次疫苗接种后14天，用血清群A菌株S276R的强毒牛莫拉菌对40头小牛进行眼部攻毒。所有未接种疫苗的小牛都患上了感染性牛角膜结膜炎（IBK）。第1组（4L株）和第2组（S276R株）的保护率分别为６０％和８０％（P小于0.05），在可能的6.0分中平均病变得分为0.7和0 .3。第3组（Maff1株）小牛的保护率仅为30％，平均病变得分为1.4，而未接种疫苗的对照为2.2。目前的研究结果，连同其他表明对IBK的免疫是血清群特异性的证据，表明在多价亚单位疫苗中包含来自澳大利亚和英国七个血清群中每个血清群的一株代表性菌株的菌毛，应能有效保护大多数牛免受澳大利亚和英国遇到的大多数牛莫拉菌野外菌株引起的IBK。
