Behnan Jinan, Stangeland Biljana, Langella Tiziana, Finocchiaro Gaetano, Tringali Giovanni, Meling Torstein R, Murrell Wayne
Department of Immunology, Norwegian Center for Stem Cell Research, Institute of Clinical Medicine, Oslo University Hospital, Oslo, Norway.
Department of Molecular Medicine, Institute of Basic Medical Sciences, The Medical Faculty, University of Oslo, Oslo, Norway.
Cell Death Dis. 2017 Aug 10;8(8):e2991. doi: 10.1038/cddis.2017.368.
Adult neural progenitor cells (aNPCs) are a potential source for cell based therapy for neurodegenerative diseases and traumatic brain injuries. These cells have been traditionally isolated from hippocampus, subventricular zone and white matter. However, there is still a need for an easily accessible source with better yield to counter the limitations of small surgical samples of previously characterized aNPCs. Here we show that ultrasonic aspirate (UA) samples currently considered as 'biological waste after surgery,' offer a good source for aNPCs. Furthermore, we show that culture conditions dictated the phenotype of cells across patients. The neurosphere-enriched cells were more similar to freshly isolated brain cells, while cells expanded adherently in serum conditions were similar to mesenchymal stem cells. However, cells expanded in these adherent conditions expressed some NPC and glial markers in addition to active canonical Wnt signaling. This suggests a mesenchymal-neuroectodermal hybrid nature of these cells. Finally, we show that UA-NPCs are comparable to those from neurogenic regions. Our findings suggest that UA samples can be used as a source for fresh and in vitro propagated aNPCs that could have various clinical applications.
成体神经祖细胞（aNPCs）是用于神经退行性疾病和创伤性脑损伤细胞疗法的潜在细胞来源。传统上，这些细胞是从海马体、脑室下区和白质中分离出来的。然而，仍需要一种易于获取且产量更高的来源，以克服先前已鉴定的aNPCs手术样本量小的局限性。在此我们表明，目前被视为“术后生物废弃物”的超声抽吸物（UA）样本，是aNPCs的良好来源。此外，我们表明培养条件决定了不同患者来源细胞的表型。富含神经球的细胞与新鲜分离的脑细胞更为相似，而在血清条件下贴壁扩增的细胞则与间充质干细胞相似。然而，在这些贴壁条件下扩增的细胞除了具有活跃的经典Wnt信号外，还表达一些NPC和神经胶质细胞标志物。这表明这些细胞具有间充质 - 神经外胚层混合特性。最后，我们表明UA - NPCs与来自神经源性区域的NPCs相当。我们的研究结果表明，UA样本可作为新鲜的和体外扩增的aNPCs的来源，这些aNPCs可能具有多种临床应用。
