利用超快DNA光交联实现DNA链置换的光化学加速
Photochemical Acceleration of DNA Strand Displacement by Using Ultrafast DNA Photo-crosslinking.
作者信息
Nakamura Shigetaka, Hashimoto Hirokazu, Kobayashi Satoshi, Fujimoto Kenzo
机构信息
School of Materials Science, Japan Advanced Institute Science and Technology, 1-1, Asahidai, Nomi, Ishikawa, 923-1292, Japan.
Department of Computer Science, University of Electro-Communications, 1-1-1, Chofugaoka, Chofu, Tokyo, 182-8585, Japan.
出版信息
Chembiochem. 2017 Oct 18;18(20):1984-1989. doi: 10.1002/cbic.201700430. Epub 2017 Aug 29.
DNA strand displacement is an essential reaction in genetic recombination, biological processes, and DNA nanotechnology. In particular, various DNA nanodevices enable complicated calculations. However, it takes time before the output is obtained, so acceleration of DNA strand displacement is required for a rapid-response DNA nanodevice. Herein, DNA strand displacement by using DNA photo-crosslinking to accelerate this displacement is evaluated. The DNA photo-crosslinking of 3-cyanovinylcarbazole ( K) was accelerated at least 20 times, showing a faster DNA strand displacement. The rate of photo-crosslinking is a key factor and the rate of DNA strand displacement is accelerated through ultrafast photo-crosslinking. The rate of DNA strand displacement was regulated by photoirradiation energy.
DNA链置换是基因重组、生物过程和DNA纳米技术中的一种基本反应。特别是，各种DNA纳米器件能够进行复杂的计算。然而，在获得输出之前需要花费时间，因此对于快速响应的DNA纳米器件而言，需要加速DNA链置换。在此，评估了通过使用DNA光交联来加速这种置换的DNA链置换。3-氰基乙烯基咔唑（K）的DNA光交联至少加速了20倍，显示出更快的DNA链置换。光交联速率是一个关键因素，并且通过超快光交联加速了DNA链置换速率。DNA链置换速率由光照射能量调节。
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