School of Advanced Science and Technology, Japan Advanced Institute Science and Technology, Asahidai 1-1, Nomi, Ishikawa, 923-1292, Japan.
Chembiochem. 2020 Nov 16;21(22):3244-3248. doi: 10.1002/cbic.202000227. Epub 2020 Jul 23.
The regulation of DNAzyme activity is an important problem for its in vivo applications. We achieved photochemical regulation of DNAzyme activity by using reversible DNA photo-crosslinking of 3-cyanovinylcarbazole ( K). The ODN containing K photo-crosslinked to a pyrimidine base in the complementary strand after a few seconds of photoirradiation, and its photoadduct was split by photoirradiation of another wavelength. The activity of photo-crosslinked DNAzyme with K was completely inhibited (OFF state). In contrast, after 312 nm irradiation, DNAzyme activity was recovered upon addition of a substrate strand (ON state). In addition, the photo-crosslinked DNAzyme is prone to enzymatic digestion by exonuclease. This photochemical OFF to ON switching with reversible DNA photo-crosslinking was regulated at the desired time and position; therefore, it might be possible to use it for in vivo application.
DNAzyme 活性的调控是其体内应用的一个重要问题。我们通过使用 3-氰基乙烯基咔唑（K）的可逆 DNA 光交联来实现 DNAzyme 活性的光化学调控。含有 K 的 ODN 在几秒钟的光照射后与互补链上的嘧啶碱基光交联，其光加合物在另一个波长的光照射下被分裂。带有 K 的光交联 DNAzyme 的活性完全被抑制（关闭状态）。相比之下，在 312nm 照射后，加入底物链后 DNAzyme 活性得到恢复（开启状态）。此外，光交联的 DNAzyme 容易被核酸外切酶消化。这种通过可逆 DNA 光交联的光化学关闭到开启的切换可以在所需的时间和位置进行调控；因此，它可能可用于体内应用。
