Ren Jie, Sang Yu, Qin Ran, Cui Zhongli, Yao Yu-Feng
Department of Microbiology & Immunology, Laboratory of Bacterial Pathogenesis, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Future Microbiol. 2017 Sep;12:1045-1057. doi: 10.2217/fmb-2017-0055. Epub 2017 Aug 10.
Acid is an important environmental condition encountered frequently by Salmonella enterica serovar Typhimurium during its pathogenesis, but the role of small-noncoding RNAs (sRNAs) in response to acid stress is poorly understood.
We used RNA sequencing to explore acid-responsive sRNAs in S. Typhimurium.
It identified that 6S RNA encoded by the ssrS was significantly upregulated at pH 3.0. The 6S RNA knockout strain showed a reduced ability to survive at pH 3.0. Additionally, genes in Salmonella pathogenicity island-1 were downregulated in the 6S RNA knockout strain. The loss of 6S RNA significantly reduced S. Typhimurium invasion ability in HeLa cells and virulence in a mouse model.
These findings demonstrate that 6S RNA plays an important role in S. Typhimurium survival under extremely acid conditions and for invasion of epithelial cells.
酸是鼠伤寒沙门氏菌在发病过程中经常遇到的一种重要环境条件，但小非编码RNA（sRNA）在应对酸应激中的作用尚不清楚。
我们使用RNA测序来探索鼠伤寒沙门氏菌中对酸有反应的sRNA。
发现在pH 3.0时，由ssrS编码的6S RNA显著上调。6S RNA基因敲除菌株在pH 3.0时的存活能力降低。此外，鼠伤寒沙门氏菌毒力岛-1中的基因在6S RNA基因敲除菌株中表达下调。6S RNA的缺失显著降低了鼠伤寒沙门氏菌在HeLa细胞中的侵袭能力以及在小鼠模型中的毒力。
这些发现表明，6S RNA在鼠伤寒沙门氏菌在极端酸性条件下的存活以及侵袭上皮细胞方面发挥着重要作用。
