Schmitz Mélodie, Ziv Tamar, Admon Arie, Baekelandt Sébastien, Mandiki Syaghalirwa N M, L'Hoir Maëlenn, Kestemont Patrick
Research Unit in Environmental and Evolutionary Biology, University of Namur, rue de Bruxelles 61, B-5000 Namur, Belgium.
The Smoler Proteomics Center, Emerson Life Science Building, Technion - Israel Institute of Technology, 32000 Haifa, Israel.
J Proteomics. 2017 Sep 7;167:12-24. doi: 10.1016/j.jprot.2017.08.005. Epub 2017 Aug 7.
In the Mekong Delta, striped catfish are faced with chronic salinity stress related to saltwater intrusion induced by global climatic changes. In this study, striped catfish juveniles were submitted to a prolonged salinity stress (up to 10ppt) over three weeks followed by infection with a virulent bacterial strain, Edwardsiella ictaluri. Osmoregulatory parameters were investigated. In addition, a label free quantitative proteomics workflow was performed on kidneys. The workflow consisted of an initial global profiling of relative peptide abundances (by LC/MS, peak area quantification based on extracted ion currents), followed by identification (by MS/MS). The aim of the study was to highlight specific functional pathways modified during realistic salinity stress, particularly those involved in immunity. In kidney proteome, 2483 proteins were identified, of which 400 proteins were differentially expressed between the freshwater and the saline water conditions. Several pathways and functional categories were highlighted, mostly related to energy metabolism, protein metabolism, actin cytoskeleton, signaling, immunity, and detoxification. In particular, the responsiveness of proteins involved in small GTPases and Mitogen Activated Protein Kinase p38 signaling, phagolysosome maturation, and T-cells regulation is discussed.
In the Mekong River Delta (Vietnam), striped catfish production is threatened by extensive sea water intrusion exacerbated by sea level rise. In fish, the effect of chronic exposure to salinity stress on immune capacities and response to disease has been poorly investigated. This study aims to highlight the main molecular changes occurring in the kidney during acclimation to salinity stress, particularly those involved in the immune defences of fish.
在湄公河三角洲，条纹鲶鱼面临着与全球气候变化导致的海水入侵相关的长期盐度胁迫。在本研究中，条纹鲶鱼幼鱼在三周内承受了长达10ppt的长期盐度胁迫，随后感染了强毒菌株鮰爱德华氏菌。研究了渗透调节参数。此外，对肾脏进行了无标记定量蛋白质组学分析流程。该流程包括首先对相对肽丰度进行整体分析（通过液相色谱/质谱联用，基于提取离子流进行峰面积定量），随后进行鉴定（通过串联质谱）。本研究的目的是突出在实际盐度胁迫期间发生改变的特定功能途径，特别是那些与免疫相关的途径。在肾脏蛋白质组中，鉴定出了2483种蛋白质，其中400种蛋白质在淡水和盐水条件之间存在差异表达。突出了几个途径和功能类别，主要与能量代谢、蛋白质代谢、肌动蛋白细胞骨架、信号传导、免疫和解毒有关。特别讨论了参与小GTP酶和丝裂原活化蛋白激酶p38信号传导、吞噬溶酶体成熟和T细胞调节的蛋白质的反应性。
在越南湄公河三角洲，条纹鲶鱼的生产受到海平面上升加剧的广泛海水入侵的威胁。在鱼类中，长期暴露于盐度胁迫对免疫能力和疾病反应的影响研究较少。本研究旨在突出在适应盐度胁迫过程中肾脏中发生的主要分子变化，特别是那些与鱼类免疫防御有关的变化。
