Institute of Solid Mechanics, Technische Universitt Braunschweig, Braunschweig D-38106, Germany.
Institute of Microtechnology, Technische Universitt Braunschweig, Braunschweig D-38124, Germany.
J Mech Behav Biomed Mater. 2018 Jan;77:764-775. doi: 10.1016/j.jmbbm.2017.07.037. Epub 2017 Jul 26.
The present study focused on the material characterisation of porcine oocytes by experiments combined with an inverse finite-element method (iFEM) approach. In doing so, two different deformation states, compression and indentation, were realised to enable the validation of the numerical model. In addition to classical force-strain relations, geometrical information on the oocyte's surface and volume changes during deformation were collected. These data reveal the typical exponential force-strain behaviour and the highly compressible behaviour of the zona pellucida, imparting overall compressibility to the entire cell. Both force-strain characteristics and geometrical information, along with different combinations of them, were used within the iFEM to identify associated material parameters. As suspected, the identified material parameters show a strong dependence on the information (force-strain relation and/or geometrical information) used in the identification process. Finally, forward finite-element calculations were applied, which verified the quality of the obtained material parameters.
本研究通过实验与逆有限元法（iFEM）相结合的方法，对猪卵母细胞的材料特性进行了研究。为此，实现了两种不同的变形状态，即压缩和压痕，以验证数值模型。除了经典的力-应变关系外，还收集了卵母细胞表面和体积在变形过程中的几何信息。这些数据揭示了典型的指数力-应变行为和透明带的高可压缩性，赋予整个细胞整体可压缩性。在 iFEM 中，使用力-应变特性和几何信息及其不同组合来识别相关的材料参数。正如预期的那样，所识别的材料参数强烈依赖于识别过程中使用的信息（力-应变关系和/或几何信息）。最后，应用正向有限元计算验证了所获得的材料参数的质量。
