肺炎克雷伯菌和鼠伤寒沙门氏菌1型菌毛亚基编码基因的核苷酸序列。
Nucleotide sequences of the genes encoding type 1 fimbrial subunits of Klebsiella pneumoniae and Salmonella typhimurium.
作者信息
Purcell B K, Pruckler J, Clegg S
机构信息
Department of Microbiology, University of Iowa, Iowa City 52242.
出版信息
J Bacteriol. 1987 Dec;169(12):5831-4. doi: 10.1128/jb.169.12.5831-5834.1987.
Abstract
The nucleotide sequences of the genes encoding the subunits of Klebsiella pneumoniae and Salmonella typhimurium type 1 fimbriae were determined. Comparison of the predicted amino acid sequences of the two subunits revealed domains in which the sequences were highly conserved. Both gene products possessed signal peptides, a fact consistent with the transport of the fimbrial subunit across the membrane, but these regions showed no amino acid homology between the two proteins. The predicted N-terminal amino acid sequences of the processed fimbrial subunits were in good agreement with those obtained by purification of the fimbrial subunits.
摘要
测定了编码肺炎克雷伯菌和鼠伤寒沙门氏菌1型菌毛亚基的基因的核苷酸序列。对这两个亚基的预测氨基酸序列进行比较，发现了序列高度保守的结构域。两种基因产物都具有信号肽，这一事实与菌毛亚基跨膜转运一致，但这两个区域在两种蛋白质之间没有氨基酸同源性。加工后的菌毛亚基的预测N端氨基酸序列与通过菌毛亚基纯化获得的序列高度一致。
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