Merrill J E, Mohlstrom C
Int Arch Allergy Appl Immunol. 1987;82(2):195-201. doi: 10.1159/000234186.
Using oligodendrocytes from primary brain cultures as targets in an antibody-dependent cellular cytotoxicity (ADCC) assay, we have examined the effects of insulin and histamine on killer cells in multiple sclerosis (MS) and other neurological disease (OND) controls compared to normal healthy controls. The effects were shown to be specific for effectors by preincubation experiments. MS patients' ADCC to primary oligodendrocytes was depressed, but could be boosted to normal control levels by histamine binding to the H1 receptor. Significant elevation of MS ADCC by cimetidine alone suggested that endogenous histamine production and H2 receptor binding could be mediating a suppressive effect on MS ADCC to oligodendrocytes. In addition, MS ADCC could be boosted significantly by insulin. MS killer cells were more sensitive in vitro to the boosting effects of both histamine and insulin than either OND or normal controls, both in dose response and magnitude of the increased ADCC.
在抗体依赖的细胞毒性（ADCC）试验中，我们使用原代脑培养中的少突胶质细胞作为靶标，研究了胰岛素和组胺对多发性硬化症（MS）患者及其他神经系统疾病（OND）对照患者的杀伤细胞的影响，并与正常健康对照进行比较。预孵育实验表明这些作用对效应细胞具有特异性。MS患者对原代少突胶质细胞的ADCC作用降低，但组胺与H1受体结合可使其增强至正常对照水平。仅西咪替丁就能显著提高MS患者的ADCC，这表明内源性组胺的产生和H2受体结合可能介导了对MS患者针对少突胶质细胞的ADCC的抑制作用。此外，胰岛素也能显著增强MS患者的ADCC。在剂量反应和ADCC增加的幅度方面，MS杀伤细胞在体外对组胺和胰岛素的增强作用比OND患者或正常对照更敏感。
