Department of Rheumatology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan 410008, People's Republic of China.
Department of Neurology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan 410008, People's Republic of China.
Biomed Pharmacother. 2017 Oct;94:697-704. doi: 10.1016/j.biopha.2017.07.088. Epub 2017 Aug 8.
Dermatomyositis (DM) is an idiopathic inflammatory myopathy characterized by CD4+ T cells and B cells infiltration in perivascular and muscle tissue. Although the infiltration of inflammatory cells plays a key role in muscle damage, the exact mechanism is not clear. Cathepsin G (CTSG) is a member of the serine proteases family and can increase the permeability of vascular endothelial cells and the chemotaxis of inflammatory cells. In this study, we found that the expression of CTSG was increased in peripheral blood mononuclear cells and muscle tissues of DM patients. The activity of CTSG was significantly increased in DM patients and correlated with disease activity. Serum CTSG induced the expression of protease activated receptor 2 (PAR2) and altered the cytoskeleton of human dermal microvascular endothelial cells. Our studies indicate, for the first time, that CTSG may play an important role in muscle inflammatory cells infiltration by increasing the permeability of vascular endothelial cells.
皮肌炎（DM）是一种特发性炎症性肌病，其特征是血管周围和肌肉组织中存在 CD4+T 细胞和 B 细胞浸润。尽管炎症细胞的浸润在肌肉损伤中起关键作用，但确切的机制尚不清楚。组织蛋白酶 G（CTSG）是丝氨酸蛋白酶家族的一员，可增加血管内皮细胞的通透性和炎症细胞的趋化性。在这项研究中，我们发现 DM 患者外周血单核细胞和肌肉组织中 CTSG 的表达增加。CTSG 的活性在 DM 患者中显著增加，且与疾病活动度相关。血清 CTSG 诱导蛋白酶激活受体 2（PAR2）的表达，并改变人真皮微血管内皮细胞的细胞骨架。我们的研究首次表明，CTSG 通过增加血管内皮细胞的通透性，可能在肌肉炎症细胞浸润中发挥重要作用。
