Jesus Flávia L M, de Almeida Fernanda B, Duarte Jonatas L, Oliveira Anna E M F M, Cruz Rodrigo A S, Souto Raimundo N P, Ferreira Ricardo M A, Kelmann Regina Gendzelevski, Carvalho José C T, Lira-Guedes Ana C, Guedes Marcelino, Solans Conxita, Fernandes Caio P
Post-Graduate Program in Tropical Biodiversity, Federal University of Amapá, Rodovia Juscelino Kubitschek, KM-02, Macapá, AP, Brazil.
Laboratory of Phytopharmaceutical Nanobiotechnology, Federal University of Amapá, Rodovia Juscelino Kubitschek KM-02, Macapá, AP, Brazil.
Evid Based Complement Alternat Med. 2017;2017:6756793. doi: 10.1155/2017/6756793. Epub 2017 Jul 17.
Andiroba () seeds are the source of an oil with a wide range of biological activities and ethnopharmacological uses. However, few studies have devoted attention to innovative formulations, including nanoemulsions. The present study aimed to obtain a colloidal system with the andiroba oil using a low-energy and organic-solvent-free method. Moreover, the preliminary residual larvicidal activity of the nanoemulsion against was evaluated. Oleic and palmitic acids were the major fatty acids, in addition to the phytosterol -sitosterol and limonoids (tetranortriterpenoids). The required hydrophile-lipophile was around 11.0 and the optimal nanoemulsion was obtained using polysorbate 85. The particle size distribution suggested the presence of small droplets (mean diameter around 150 nm) and low polydispersity index (around 0.150). The effect of temperature on particle size distribution revealed that no major droplet size increase occurred. The preliminary residual larvicidal assay suggested that the mortality increased as a function of time. The present study allowed achievement of a potential bioactive oil in water nanoemulsion that may be a promising controlled release system. Moreover, the ecofriendly approach involved in the preparation associated with the great bioactive potential of makes this nanoemulsion very promising for valorization of this Amazon raw material.
香豆树（）种子是一种具有广泛生物活性和民族药理学用途的油的来源。然而，很少有研究关注创新制剂，包括纳米乳液。本研究旨在使用低能量且无有机溶剂的方法获得一种含有香豆树油的胶体体系。此外，还评估了该纳米乳液对的初步残留杀幼虫活性。除了植物甾醇 - 谷甾醇和柠檬苦素（四降三萜类化合物）外，油酸和棕榈酸是主要脂肪酸。所需的亲水亲油平衡值约为11.0，使用聚山梨酯85获得了最佳纳米乳液。粒度分布表明存在小液滴（平均直径约150nm）且多分散指数较低（约0.150）。温度对粒度分布的影响表明液滴尺寸没有显著增加。初步残留杀幼虫试验表明死亡率随时间增加。本研究成功制备了一种潜在的水包油型生物活性纳米乳液，它可能是一种很有前景的控释系统。此外，制备过程中采用的环保方法以及香豆树巨大的生物活性潜力，使得这种纳米乳液在这种亚马逊原材料的增值利用方面非常有前景。
