Kim Dong-Uk, Lee Hyosun, Lee Suyeon, Park Sooyeon, Yoon Jung-Hoon, Park So Yoon, Ka Jong-Ok
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Arch Microbiol. 2018 Jan;200(1):91-96. doi: 10.1007/s00203-017-1424-8. Epub 2017 Aug 10.
A Gram-stain-negative and yellow-pigmented bacterial strain, designated TX0653, was isolated from an automobile evaporator core collected in Korea. The cells were aerobic and rod-shaped. The strain grew at 10-28 °C (optimum, 25 °C), at pH 6.0-7.5 (optimum, 6.5), and in the presence of 0-1% (w/v) NaCl (optimum, 0%). Phylogenetically, the strain was related to members of the genus Spirosoma (95.1-90.8% 16S rRNA sequence similarity) and distantly related to Spirosoma pulveris JSH5-14 (95.1%), Spirosoma fluviale MSd3 (95.0%), Spirosoma endophyticum DSM 26130 (94.8%), and Spirosoma linguale DSM 74 (94.6%). The major fatty acids of the strain were summed feature 3 (C ω6c and/or C ω7c), C ω5c, iso-C, iso-C 3-OH, and C. MK-7 was identified as the predominant menaquinone. The polar lipids profile indicated the presence of one phosphatidylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid, two unidentified phospholipids, and three unidentified lipids. On the basis of the phenotypic, genotypic, and chemotaxonomic characteristics, strain TX0653 represents a novel species in the genus Spirosoma, for which the name Spirosoma metallum sp. nov. (= KACC 19278 = NBRC 112495) is proposed.
从韩国收集的汽车蒸发器芯中分离出一株革兰氏阴性、产黄色色素的细菌菌株，命名为TX0653。细胞为需氧型，呈杆状。该菌株在10-28℃（最适温度25℃）、pH值6.0-7.5（最适pH值6.5）以及0-1%（w/v）NaCl（最适浓度0%）条件下生长。系统发育分析表明，该菌株与螺菌属成员相关（16S rRNA序列相似度为95.1-90.8%），与粉状螺菌JSH5-14（95.1%）、河流螺菌MSd3（95.0%）、内生螺菌DSM 26130（94.8%）和舌螺菌DSM 74（94.6%）亲缘关系较远。该菌株的主要脂肪酸为特征性总和3（C ω6c和/或C ω7c）、C ω5c、异-C、异-C 3-OH和C。MK-7被鉴定为主要的甲基萘醌。极性脂质谱显示存在一种磷脂酰乙醇胺、一种未鉴定的氨基脂质、一种未鉴定的氨基磷脂、两种未鉴定的磷脂和三种未鉴定的脂质。基于表型、基因型和化学分类学特征，菌株TX0653代表螺菌属中的一个新物种，为此提出了新名称金属螺菌（Spirosoma metallum sp. nov.）（=KACC 19278=NBRC 112495）。
