Kim Dong-Uk, Lee Hyosun, Lee Suyeon, Park Sooyeon, Yoon Jung-Hoon, Park So Yoon, Ka Jong-Ok
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Oct;67(10):4195-4199. doi: 10.1099/ijsem.0.002276. Epub 2017 Sep 18.
A Gram-stain-negative and yellow-pigmented bacterial strain, designated TX0406, was isolated from an automobile evaporator core collected in Korea. The cells were non-motile, aerobic and rod-shaped. The strain grew at 15-37 °C (optimum, 25 °C), at pH 6.0-7.0 (optimum, 6.5) and in the presence of 0-1.5 % (w/v) NaCl. Phylogenetically, the strain was related to members of the genus Spirosoma(93.7-90.7 % 16S rRNA sequence similarities) and showed the highest sequence similarity of 93.7 % to Spirosomapulveris JSH5-14. The major fatty acids of the strain were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 1ω5c and C16 : 0. The predominant menaquinone was MK-7. The polar lipid profile revealed the presence of phosphatidylethanolamine, an unidentified aminolipid, unidentified aminophospholipids and unidentified lipids. The DNA G+C content of the strain was 58.7 mol%. Based on phenotypic, genotypic and chemotaxonomic data, strain TX0406 represents a novel species in the genus Spirosoma, for which the name Spirosoma carri sp. nov. (=KACC 19013=NBRC 112494) is proposed.
从韩国收集的汽车蒸发器芯中分离出一株革兰氏染色阴性且产黄色色素的细菌菌株，命名为TX0406。该细胞无运动性，好氧，呈杆状。该菌株在15 - 37°C（最适温度为25°C）、pH 6.0 - 7.0（最适pH为6.5）以及0 - 1.5%（w/v）NaCl存在的条件下生长。在系统发育上，该菌株与螺菌属成员相关（16S rRNA序列相似性为93.7 - 90.7%），与粉状螺菌JSH5 - 14的序列相似性最高，为93.7%。该菌株的主要脂肪酸为总和特征3（C16 : 1ω7c和/或C16 : 1ω6c）、C16 : 1ω5c和C16 : 0。主要的甲基萘醌为MK - 7。极性脂质谱显示存在磷脂酰乙醇胺、一种未鉴定的氨基脂质、未鉴定的氨基磷脂和未鉴定的脂质。该菌株的DNA G + C含量为58.7 mol%。基于表型、基因型和化学分类学数据，菌株TX0406代表螺菌属中的一个新物种，为此提出名为Carri螺菌新种（Spirosoma carri sp. nov.）（=KACC 19013=NBRC 112494）。
