Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
J Microbiol. 2017 Sep;55(9):673-677. doi: 10.1007/s12275-017-7162-4. Epub 2017 Aug 5.
A Gram-stain-negative and orangish yellow-pigmented bacterial strain, designated PR1014K, was isolated from an automobile evaporator core collected in Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PR1014K was related with the members of the genus Spirosoma (94.7-90.2%) and closely related with Spirosoma lacussanchae CPCC 100624 (94.7%), Spirosoma knui 15J8-12 (94.3%), and Spirosoma soli MIMBbqt12 (93.3%). The strain grew at 15-40°C (optimum, 25°C), pH 6.5-7.0 (optimum, 6.5) and 0-1% (w/v) NaCl (optimum, 0%). The predominant fatty acids were summed feature 3 (C ω7c and/or C ω6c), C, iso-C, C ω5c, and iso-C 3-OH. The major menaquinone was MK-7. The polar lipid profile of the strain indicated that the presence of one phosphatidylethanolamine, one unidentified aminolipid, two unidentified aminophospholipids, and three unidentified lipids. The DNA G+C content of the strain was 47.4 mol%. On the basis of the phenotypic, genotypic and chemotaxonomic characteristics, strain PR1014K represents a novel species in the genus Spirosoma, for which the name Spirosoma metallicus sp. nov. (=KACC 17940 =NBRC 110792) is proposed.
一株革兰氏阴性、橙黄色素的细菌菌株，被命名为 PR1014K，从韩国采集的汽车蒸发器芯中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 PR1014K 与 Spirosoma 属的成员（94.7-90.2%）有关，与 Spirosoma lacussanchae CPCC 100624（94.7%）、Spirosoma knui 15J8-12（94.3%）和 Spirosoma soli MIMBbqt12（93.3%）最为密切相关。该菌株在 15-40°C（最适温度 25°C）、pH6.5-7.0（最适 pH6.5）和 0-1%（w/v）NaCl（最适 0%）的条件下生长。主要脂肪酸为 summed feature 3（C ω7c 和/或 C ω6c）、C 14:0、iso-C 15:0、C ω5c 和 iso-C 3-OH。主要的menaquinone 是 MK-7。该菌株的极性脂谱表明，存在一个磷脂酰乙醇胺、一个未鉴定的胺脂、两个未鉴定的胺磷脂和三个未鉴定的脂类。该菌株的 DNA G+C 含量为 47.4 mol%。根据表型、基因型和化学分类学特征，菌株 PR1014K 代表了 Spirosoma 属中的一个新种，建议将其命名为 Spirosoma metallicus sp. nov.（=KACC 17940 =NBRC 110792）。
