Department of Diagnostic Sciences, Tufts University School of Dental Medicine, Boston, MA, USA.
Department of Diagnostic Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
Mod Pathol. 2017 Dec;30(12):1646-1654. doi: 10.1038/modpathol.2017.71. Epub 2017 Aug 11.
Human papillomavirus (HPV) 16 is the most common high-risk HPV type identified in oropharyngeal and cervical neoplasia. Recently, HPV-associated oral epithelial dysplasia with specific histopathologic features and demographics similar to HPV-oropharyngeal carcinoma has been identified. The objective of this study was to evaluate histopathologically all cases of HPV-oral epithelial dysplasia seen in one center and identify HPV types in a subset of cases. Cases with specific histopathology for HPV-oral epithelial dysplasia that were positive both by immunohistochemical studies for p16 and by in situ hybridization for high-risk types of HPV were further analyzed using QIAamp DNA Tissue Kits (Qiagen, Hilden, Germany). DNA was extracted, amplified, and digested with restriction enzymes and run on a polyacrylamide gel. Digestion patterns were visually compared with a database of known HPV digestion patterns for identification. There were 53 specimens included in the analysis. There were 47 males and six females (7.8:1), with a median age of 55 years (range 41-81). The most common site of involvement was the tongue/floor of mouth (77% of cases). Of the 53 cases, 94% exhibited parakeratosis and/or hyperkeratosis. All the cases featured karyorrhexis, apoptosis, and characteristics of conventional carcinoma in situ. The quantity of DNA extracted was sufficient for analysis in 22 cases. HPV-16 was identified in 20/22 (91%) cases. One case was associated with HPV-33 and one with HPV-58 (5% each). Eight of the 53 cases (15%) were associated with invasive squamous cell carcinomas.
人乳头瘤病毒（HPV）16 是口咽和宫颈癌中最常见的高危 HPV 类型。最近，已经确定了具有特定组织病理学特征和与 HPV 口咽癌相似的人口统计学特征的 HPV 相关口腔上皮异型增生。本研究的目的是评估一个中心所见的所有 HPV 口腔上皮异型增生的组织病理学，并确定一部分病例中的 HPV 类型。对具有 HPV 口腔上皮异型增生特定组织病理学特征且免疫组织化学研究 p16 阳性且原位杂交检测高危型 HPV 阳性的病例，使用 QIAamp DNA 组织试剂盒（Qiagen，德国希尔德斯海姆）进一步分析。提取、扩增 DNA，并使用限制性内切酶进行消化，然后在聚丙烯酰胺凝胶上运行。通过视觉比较消化模式与已知 HPV 消化模式数据库来识别 HPV 类型。分析中包括 53 个标本。有 47 名男性和 6 名女性（7.8:1），中位年龄为 55 岁（范围 41-81）。最常见的受累部位是舌/口底（77%的病例）。在 53 例中，94%表现为角化过度和/或过度角化。所有病例均具有核碎裂、细胞凋亡和原位癌的特征。22 例中提取的 DNA 量足以进行分析。在 20/22（91%）例中鉴定出 HPV-16。1 例与 HPV-33 相关，1 例与 HPV-58 相关（各占 5%）。53 例中有 8 例（15%）与浸润性鳞状细胞癌相关。
