Department of Pathology, School of Medicine, University of Milan-Bicocca, Italy.
Anticancer Res. 2010 Sep;30(9):3435-40.
Human papillomavirus DNA (HPV DNA) and p16 and p53 protein expressions were investigated for their role in transforming dysplasia into squamous cell carcinoma of the oral cavity in a non-smoker and non-drinker patient group.
A total of 56 oral biopsies from non-smoker and non-drinker patients were analyzed. The specimens were grouped into three categories: group 1 included 31 cases of hyperplastic mucosa and mild dysplasia, group 2 included 14 cases of moderate and severe dysplasia, while group 3 comprised 11 cases of invasive squamous cell carcinomas. In all cases, immunohistochemical methods were performed to detect p16 and p53 protein expressions. The nested polymerase chain reaction for HPV (nested HPV-PCR) and the catalyzed signal-amplified colorimetric DNA in situ hybridization (CSAC-ISH) methods were applied for HPV DNA detection and typing of high-risk genotype.
P16 protein, absent from all specimens of group 1, was especially noted in group 2 (92.86%) and in group3 (54.55%). Five out of 14 of group 2 cases (35.71%) and 3/11 (27.27%) of group 3 were HPV DNA positive. The HPVs detected were of both high-risk and low-risk genotype. The analysis of the relationship between HPV and p16 protein expression revealed that all the group 2 and 3 samples with HPV DNA, overexpressed p16 protein.
The results suggest that HPV could be a molecular marker in group 2 and 3 specimens in non-smoker and non-drinker patients. The virus may play an etiological role in carcinogenesis in the oral cavity. The association between HPV and p16 overexpression suggests a molecular mechanism similar to that found in cervical cancer.
在非吸烟和非饮酒患者群体中，研究了人乳头瘤病毒 DNA（HPV DNA）和 p16 和 p53 蛋白表达在将口腔发育不良转化为鳞状细胞癌中的作用。
分析了 56 例非吸烟和非饮酒患者的口腔活检标本。这些标本分为三组：第 1 组包括 31 例增生性黏膜和轻度发育不良，第 2 组包括 14 例中度和重度发育不良，第 3 组包括 11 例浸润性鳞状细胞癌。所有病例均采用免疫组织化学方法检测 p16 和 p53 蛋白表达。应用巢式聚合酶链反应（nested HPV-PCR）和催化信号放大比色原位杂交（CSAC-ISH）方法检测 HPV DNA 并对高危基因型进行分型。
p16 蛋白在第 1 组所有标本中均不存在，在第 2 组（92.86%）和第 3 组（54.55%）中特别明显。第 2 组 14 例中有 5 例（35.71%）和第 3 组 11 例中有 3 例（27.27%）HPV DNA 阳性。检测到的 HPV 既有高危型也有低危型。HPV 与 p16 蛋白表达的关系分析表明，第 2 组和第 3 组所有 HPV DNA 阳性标本均过度表达 p16 蛋白。
结果表明，HPV 可能是非吸烟和非饮酒患者第 2 组和第 3 组标本的分子标志物。该病毒可能在口腔癌的发生中起病因作用。HPV 与 p16 过度表达的关联提示存在类似于宫颈癌的分子机制。
