Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, Taiwan.
Nanoscale. 2017 Aug 24;9(33):12087-12095. doi: 10.1039/c7nr03431h.
Herein, we report ultra-sensitive sensing of a prostate-specific antigen (PSA), which is used as a biomarker to detect prostate cancer, using a molybdenum series (MoO, MoS, and MoSe) of two-dimensional nanosheets (2D NSs). Moreover, the design of a 2D NS-based PSA aptamer sensor system was demonstrated based on a fluorescence turn-on mechanism in the presence of a target. The 2D NSs acted as an excellent sensing platform in which the PSA aptamer was adsorbed on the NSs and subsequent energy transfer between them led to fluorescence quenching of the aptamer. The detection limit of PSA was achieved to be 13 pM for MoO NSs, whereas the MoS and MoSe systems exhibited a detection limit of 72 and 157 pM, respectively. To the best of our knowledge, this is the first report on the ultra-sensitive detection of a 2D NS-based aptamer sensor. The in vitro bioimaging measurements were performed using confocal fluorescence microscopy. Herein, PSA detection was successfully demonstrated in human embryonic kidney 293T (HEK) live cells. Moreover, the MoO, MoS, and MoSe NSs exhibit excellent biocompatibility and low toxicity; thus, these 2D NSs can be used as a promising sensor platform to detect prostate cancer.
本文报道了一种使用二维纳米片（2D NS）的钼系列（MoO、MoS 和 MoSe）对前列腺特异性抗原（PSA）进行超灵敏检测的方法，PSA 是一种用于检测前列腺癌的生物标志物。此外，还基于靶标存在下的荧光开启机制，展示了基于 2D NS 的 PSA 适体传感器系统的设计。2D NS 作为一种优异的传感平台，PSA 适体被吸附在 NS 上，随后它们之间的能量转移导致适体的荧光猝灭。MoO NSs 的 PSA 检测限达到 13 pM，而 MoS 和 MoSe 体系的检测限分别为 72 pM 和 157 pM。据我们所知，这是首例关于基于 2D NS 的适体传感器超灵敏检测的报道。体外生物成像测量使用共聚焦荧光显微镜进行。本文成功地在人胚肾 293T（HEK）活细胞中进行了 PSA 检测。此外，MoO、MoS 和 MoSe NSs 具有优异的生物相容性和低毒性；因此，这些 2D NS 可用作检测前列腺癌的有前途的传感器平台。
