Seoane Marta, Esperanza Marta, Cid Ángeles
Laboratorio de Microbiología, Facultad de Ciencias, Universidade da Coruña, Campus da Zapateira s/n, 15071, A Coruña, Spain.
Laboratorio de Microbiología, Facultad de Ciencias, Universidade da Coruña, Campus da Zapateira s/n, 15071, A Coruña, Spain.
Aquat Toxicol. 2017 Oct;191:62-72. doi: 10.1016/j.aquatox.2017.08.001. Epub 2017 Aug 2.
Omeprazole (OMP) is one of the most commonly used drugs for the treatment of gastro-intestinal disorders. Although it is daily consumed in high quantities and commonly detected in waters worldwide, relatively little is known about its ecotoxicity. The aim of this study was to evaluate the potential acute toxicity of increasing concentrations of OMP on the marine microalga Tetraselmis suecica analysing several cytotoxicity biomarkers by flow cytometry after 24h of exposure. Results showed that OMP caused a decrease in growth and autofluorescence, an increase in cellular volume and intracellular complexity, hyperpolarization of cytoplasmic and mitochondrial membranes and intracellular acidification. In addition, large amounts of reactive oxygen species (ROS) were generated which resulted in a decrease in the percentage of the viable population. However, the viable population showed an increase in their metabolic activity as an early response to overcome the stress. In conclusion, OMP may affect proton pumps in non-target organisms such as microalgae; it disturbed pH homeostasis and provoked an early accumulation of ROS that resulted in a rapid cell death in cells exposed to the highest concentration assayed.
奥美拉唑（OMP）是治疗胃肠道疾病最常用的药物之一。尽管它每天的消耗量很大，并且在全球范围内的水体中普遍被检测到，但人们对其生态毒性的了解相对较少。本研究的目的是通过流式细胞术分析暴露24小时后几种细胞毒性生物标志物，评估不同浓度递增的OMP对海洋微藻瑞典四爿藻的潜在急性毒性。结果表明，OMP导致生长和自发荧光降低、细胞体积和细胞内复杂性增加、细胞质和线粒体膜超极化以及细胞内酸化。此外，产生了大量活性氧（ROS），导致存活群体百分比下降。然而，存活群体表现出代谢活性增加，作为克服应激的早期反应。总之，OMP可能会影响非靶标生物（如微藻）中的质子泵；它扰乱了pH稳态，引发了ROS的早期积累，导致暴露于所检测最高浓度的细胞迅速死亡。
