Seoane Marta, Rioboo Carmen, Herrero Concepción, Cid Ángeles
Laboratorio de Microbiología, Facultad de Ciencias, Universidad de A Coruña, Campus de A Zapateira s/n, 15071 A Coruña, Spain.
Laboratorio de Microbiología, Facultad de Ciencias, Universidad de A Coruña, Campus de A Zapateira s/n, 15071 A Coruña, Spain.
Mar Environ Res. 2014 Oct;101:1-7. doi: 10.1016/j.marenvres.2014.07.011. Epub 2014 Aug 7.
Aquaculture facilities are a potential source of antibiotics to the aquatic ecosystems. The presence of these compounds in the environment may have deleterious effects on non-target aquatic organisms such as microalgae, which are often used as biological indicators of pollution. Therefore, the toxicity induced by chloramphenicol (CHL), florphenicol (FLO) and oxytetracycline (OTC), three antibiotics widely used in aquaculture, on the marine microalga Tetraselmis suecica was evaluated. Growth inhibition and physiological and biochemical parameters were analysed. All three antibiotics inhibited growth of T. suecica with 96 h IC50 values of 11.16, 9.03 and 17.25 mg L(-1) for CHL, FLO and OTC, respectively. After 24 h of exposure no effects on growth were observed and cell viability was also unaffected, whereas a decrease in esterase activity, related with cell vitality, was observed at the higher concentrations assayed. Photosynthesis related parameters such as chlorophyll a cellular content and autofluorescence were also altered after 24 h of antibiotics addition. It can be concluded that T. suecica was sensitive to the three antibiotics tested.
水产养殖设施是水生生态系统中抗生素的一个潜在来源。这些化合物在环境中的存在可能会对非目标水生生物产生有害影响，例如微藻，微藻常被用作污染的生物指标。因此，评估了水产养殖中广泛使用的三种抗生素氯霉素（CHL）、氟苯尼考（FLO）和土霉素（OTC）对海洋微藻瑞典四爿藻的毒性。分析了生长抑制以及生理和生化参数。所有三种抗生素均抑制了瑞典四爿藻的生长，CHL、FLO和OTC的96小时半数抑制浓度（IC50）值分别为11.16、9.03和17.25毫克/升。暴露24小时后，未观察到对生长的影响，细胞活力也未受影响，而在测定的较高浓度下，观察到与细胞活力相关的酯酶活性降低。添加抗生素24小时后，与光合作用相关的参数，如叶绿素a细胞含量和自发荧光也发生了变化。可以得出结论，瑞典四爿藻对所测试的三种抗生素敏感。
