Department of Basic Research on Social Recognition and Memory, Research Centre for Child Mental Development, Kanazawa University, Kanazawa, 920-8640, Japan.
Departments of Biochemistry and Molecular Vascular Biology, Kanazawa University Graduate School of Medical Sciences, Kanazawa, 920-8640, Japan.
Sci Rep. 2017 Aug 11;7(1):7883. doi: 10.1038/s41598-017-07949-4.
Plasma oxytocin (OT) originates from secretion from the pituitary gland into the circulation and from absorption of OT in mother's milk into the blood via intestinal permeability. However, the molecular mechanism underlying the absorption of OT remains unclear. Here, we report that plasma OT concentrations increased within 10 min after oral delivery in postnatal day 1-7 mice. However, in Receptors for Advanced Glycation End Products (RAGE) knockout mice after postnatal day 3, an identical OT increase was not observed. In adult mice, plasma OT was also increased in a RAGE-dependent manner after oral delivery or direct administration into the intestinal tract. Mass spectrometry evaluated that OT was absorbed intact. RAGE was abundant in the intestinal epithelial cells in both suckling pups and adults. These data highlight that OT is transmitted via a receptor-mediated process with RAGE and suggest that oral OT supplementation may be advantageous in OT drug development.
血浆催产素（OT）来源于垂体分泌到血液中，以及通过肠道通透性从母乳中吸收 OT 进入血液。然而，OT 吸收的分子机制尚不清楚。在这里，我们报告说，在出生后 1-7 天的小鼠中，口服给药后 10 分钟内血浆 OT 浓度增加。然而，在出生后第 3 天的晚期糖基化终产物受体（RAGE）敲除小鼠中，未观察到相同的 OT 增加。在成年小鼠中，口服给药或直接给予肠道后，OT 也以 RAGE 依赖的方式增加。质谱评估发现 OT 被完整吸收。RAGE 在哺乳期幼鼠和成年小鼠的肠上皮细胞中含量丰富。这些数据突出表明，OT 通过 RAGE 介导的受体途径传递，并表明口服 OT 补充可能有利于 OT 药物的开发。
