Department of Rheumatology and Clinical Immunology, University General Hospital of Larissa, Faculty of Medicine, School of Health Sciences, University of Thessaly, Viopolis, 40500, Larissa, Greece.
Department of Neurology, University General Hospital of Larissa, Faculty of Medicine, School of Health Sciences, University of Thessaly, 40500, Larissa, Greece.
Sci Rep. 2017 Aug 11;7(1):7929. doi: 10.1038/s41598-017-07801-9.
To assess whether Helicobacter pylori (Hp) antibody (ab) reactivity against individual Hp antigens is pathogenetically relevant to multiple sclerosis (MS), we systematically investigated prevalence and clinical significance of abs against 14 immunodominant and subdominant Hp antigens by ELISA and immunoblotting in 139 consecutive MS patients with relapsing-remitting (RRMS, n = 102) or secondary progressive (SPMS, n = 37). Sera from 39 patients with Parkinson's disease (PD), 21 with Alzheimer's disease (ALZ) and 68 healthy controls (HCs), were also tested. Anti-flagellin (18.3%) and anti-p41 (25.0%) abs in MS were less frequent than in HCs (39.4%, 48.5%, respectively). Abs against 5 of the 14 antigens were less frequent in RRMS than HCs, including p41, p54-flagellin, p29-UreA, p67-FSH, and p120-CagA. Anti-VacA abs were more frequent in SPMS than in HCs (42.1 vs 12.1%, p = 0.019). Anti-p54, anti-p29-UreA and anti-p26 correlated with extended disability status scale (EDSS) (p = 0.017, p = 0.005, p = 0.002, respectively). Anti-p26 and anti-p17 correlated with the number of relapses (p = 0.037 and p = 0.047, respectively). This is the first comprehensive analysis of ab reactivities against most Hp antigens in MS patients. Ab responses differ between MS and HCs and between RRMS and SPMS, being more prevalent in SPMS than RRMS, thus suggesting an association between anti-Hp and the former type of MS.
为了评估幽门螺杆菌（Hp）抗体（Ab）针对个别 Hp 抗原的反应性与多发性硬化症（MS）是否具有发病相关性，我们通过 ELISA 和免疫印迹法系统地检测了 139 例连续的 MS 患者（RRMS，n=102；SPMS，n=37）中 14 种免疫显性和亚显性 Hp 抗原的 Ab 流行率和临床意义。还检测了 39 例帕金森病（PD）患者、21 例阿尔茨海默病（ALZ）患者和 68 例健康对照者（HCs）的血清。MS 患者中的抗鞭毛蛋白（18.3%）和抗 p41（25.0%）Ab 比 HCs 少（分别为 39.4%、48.5%）。RRMS 患者中，有 5 种抗原的 Ab 比 HCs 少，包括 p41、p54-鞭毛蛋白、p29-UreA、p67-FSH 和 p120-CagA。SPMS 患者中的抗 VacA Ab 比 HCs 更频繁（42.1%比 12.1%，p=0.019）。抗 p54、抗 p29-UreA 和抗 p26 与扩展残疾状况量表（EDSS）相关（p=0.017、p=0.005、p=0.002，分别）。抗 p26 和抗 p17 与复发次数相关（p=0.037 和 p=0.047，分别）。这是首次对 MS 患者中大多数 Hp 抗原的 Ab 反应进行全面分析。Ab 反应在 MS 患者和 HCs 之间以及 RRMS 和 SPMS 之间存在差异，在 SPMS 中比 RRMS 更常见，因此提示抗 Hp 与前一种 MS 类型之间存在关联。
