Patrikiou Eleni, Efthymiou George, Liaskos Christos, Ntavari Niki, Scheper Thomas, Meyer Wolfgang, Simopoulou Theodora, Dardiotis Efthymios, Roussaki-Schulze Aggeliki-Victoria, Zafiriou Efterpi, Bogdanos Dimitrios P, Sakkas Lazaros I
Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, 41110, Greece.
Department of Dermatology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece.
Immunol Res. 2024 Dec 20;73(1):22. doi: 10.1007/s12026-024-09569-3.
Helicobacter pylori (Hp) has been postulated as an infectious trigger of psoriatic disease, namely psoriasis (Ps) and psoriatic arthritis (PsA), but meticulous antibody (ab) reactivity against all dominant and subdominant Hp antigens in demographically matched PsA and Ps patients and healthy controls has not been performed so far. IgG anti-Hp ab testing was performed by combining immunoblotting and line assays in 263 serum samples from 89 patients with PsA, 114 patients with Ps, and 60 demographically matched healthy controls (HCs). Anti-Hp positivity did not differ between PsA, Ps, and HCs (P > 0.05 for all comparisons). In PsA, anti-p75, anti-p67-FSH, anti-p66-UreB, anti-p54-flagellin, anti-p41, and anti-p30-OMP abs were more frequent in patients compared to HCs (P < 0.001, P = 0.028, P = 0.010, P = 0.003, P = 0.012, P = 0.020 respectively). In Ps, anti-p66-UreB and anti-p54-flagellin abs were more frequent than HC (P = 0.015 and P = 0.011, respectively), while anti-p50 abs were less frequent than HCs (P = 0.008). Anti-p75, anti-p67-FSH, anti-p50, anti-p41, anti-p30-OMP, anti-p29 = UreA and anti-p26 ab levels were higher in PsA compared to Ps (P = 0.012, P = 0.036, P < 0.001, P = 0.021, P = 0.002, P = 0.006 and P = 0.021 respectively). DAS28 scores were positively correlated with anti-p19 ab levels (r = 0.349, P = 0.050) in PsA patients by linear regression analysis. No other significant clinical association with anti-Hp responses was noted in patients with PsA and Ps. Our results demonstrate that several antigen-specific anti-Hp abs are more frequent in patients with psoriatic disease; however, negative correlations also exist, raising doubts about whether Hp is immunologically linked to psoriatic disease.
幽门螺杆菌（Hp）被认为是银屑病性疾病（即银屑病（Ps）和银屑病关节炎（PsA））的感染触发因素，但迄今为止，尚未对人口统计学匹配的PsA和Ps患者及健康对照者针对所有主要和次要Hp抗原的细致抗体（ab）反应性进行检测。通过免疫印迹法和线性分析相结合，对来自89例PsA患者、114例Ps患者和60例人口统计学匹配的健康对照者（HCs）的263份血清样本进行了抗Hp IgG抗体检测。PsA、Ps患者和HCs之间的抗Hp阳性率没有差异（所有比较的P>0.05）。在PsA患者中，与HCs相比，抗p75、抗p67-FSH、抗p66-UreB、抗p54-鞭毛蛋白、抗p41和抗p30-OMP抗体在患者中更常见（分别为P<0.001、P=0.028、P=0.010、P=0.003、P=0.012、P=0.020）。在Ps患者中，抗p66-UreB和抗p54-鞭毛蛋白抗体比HCs更常见（分别为P=0.015和P=0.011），而抗p50抗体比HCs更少见（P=0.008）。与Ps患者相比，PsA患者的抗p75、抗p67-FSH、抗p50、抗p41、抗p30-OMP、抗p29 = UreA和抗p26抗体水平更高（分别为P=0.012、P=0.036、P<0.001、P=0.021、P=0.002、P=0.006和P=0.021）。通过线性回归分析，PsA患者的疾病活动评分（DAS28）与抗p19抗体水平呈正相关（r=0.349，P=0.050）。在PsA和Ps患者中，未发现与抗Hp反应有其他显著的临床关联。我们的结果表明，几种抗原特异性抗Hp抗体在银屑病性疾病患者中更常见；然而，也存在负相关，这使人怀疑Hp是否与银屑病性疾病存在免疫联系。
