在免疫结合测定中使用与表位标签融合的高斯荧光素酶的多重功能生物发光报告基因。

Multiplex functional bioluminescent reporters using Gaussia luciferase fused to epitope tags in an immunobinding assay.

作者信息

van Rijn Sjoerd, Würdinger Thomas, Nilsson Jonas

机构信息

Department of Neurosurgery, Cancer Center Amsterdam, Neuro-oncology Research Group, VU University Medical Center, Amsterdam, The Netherlands.

出版信息

Methods Mol Biol. 2014;1098:231-47. doi: 10.1007/978-1-62703-718-1_18.

DOI:10.1007/978-1-62703-718-1_18

PMID:24166381

Abstract

The use of Gaussia luciferase in a multiplex assay can have several advantages over the singleplex method for an experimental setup. Issues such as intersample variability, screening purposes, efficiency, and in vivo applications can be addressed using a multiplex assay. Here we describe a functional reporter multiplex method using Gaussia luciferase fused to epitope tags to identify the different reporters that are expressed. Tag specific antibodies are used to bind and separate the tagged luciferase reporters.

摘要

在多重分析中使用高斯荧光素酶相对于单重方法用于实验设置可能具有几个优点。诸如样本间变异性、筛选目的、效率和体内应用等问题可以通过多重分析来解决。在这里，我们描述了一种功能性报告基因多重方法，该方法使用与表位标签融合的高斯荧光素酶来鉴定表达的不同报告基因。标签特异性抗体用于结合和分离带标签的荧光素酶报告基因。