Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n. Col. Casco de Santo Tomás, Miguel Hidalgo 11340 Ciudad de México, Mexico.
División Académica de Ciencias de la Salud, Universidad Juárez Autónoma de Tabasco, Avenida Gregorio Méndez Magaña 2838-A, Col. Tamulte, 86150 Villahermosa, Tabasco, Mexico.
Biomed Pharmacother. 2017 Oct;94:858-864. doi: 10.1016/j.biopha.2017.07.166. Epub 2017 Aug 10.
Syzygium aromaticum (L.) Merr. & L.M. Perry (Mirtaceae) and Rosmarinus officinalis L. (Lamiaceae) are both medicinal plants used for centuries to alleviate pain. The aim of the study was to demonstrate the therapeutic potential utility of herb-drug association of S. aromaticum essential oil or R. officinalis ethanolic extract coadministered with ketorolac. Antinociceptive pharmacological interaction was investigated by an isbolographic study using the formalin test in rats. Both alone and in combination with ketorolac; S. aromaticum and R. officinalis produced a dose-dependent antinociceptive response. To plot the isobologram, we used the effective dose 50 of each one component in a fixed 1:1 ratio. The isobolographic analysis showed that, in both combinations, ketorolac plus essential oil S. aromaticum and ketorolac plus ethanolic extract R. officinalis, the experimental value (Z) was lower than the theoretical value (Z). In addition, this study shows that eugenol, a metabolite present in S. aromaticum, and ursolic acid, a metabolite present in R. officinalis, also synergized the antinociceptive effect of ketorolac. While, the oleanolic acid present in both medicinal species did not show a synergistic antinociceptive effect in combination with ketorolac. No adverse effects were observed with these herb-drug interactions. These findings suggest that essential oil S. aromaticum and ethanolic extract R. officinalis could be useful in combination with ketorolac for the treatment of inflammatory pain.
丁香 Syzygium aromaticum（L.）Merr. & L.M. Perry（桃金娘科）和迷迭香 Rosmarinus officinalis L.（唇形科）都是药用植物，几个世纪以来一直被用于缓解疼痛。本研究旨在证明丁香精油或迷迭香乙醇提取物与酮咯酸联合使用的草药-药物联合的治疗潜力。通过在大鼠福尔马林试验中使用等辐射研究来研究抗伤害性的药理学相互作用。丁香精油和迷迭香乙醇提取物单独使用以及与酮咯酸联合使用时，均产生剂量依赖性的镇痛反应。为了绘制等辐射图，我们使用每个成分的有效剂量 50 在固定的 1:1 比例。等辐射分析表明，在这两种组合中，酮咯酸加丁香精油和酮咯酸加迷迭香乙醇提取物，实验值（Z）低于理论值（Z）。此外，这项研究表明，丁香精油中的代谢产物丁香酚和迷迭香乙醇提取物中的代谢产物熊果酸也协同了酮咯酸的镇痛作用。而两种药用植物中都存在的齐墩果酸则没有与酮咯酸表现出协同的镇痛作用。这些草药-药物相互作用没有观察到不良反应。这些发现表明，丁香精油和迷迭香乙醇提取物可能与酮咯酸联合用于治疗炎症性疼痛。
