Department of Physiology, School of Medical Sciences, Tarbiat Modares University, PO Box: 14115-331, Tehran, Iran.
Cellular and Molecular Research Center, Department of Physiology and Pharmacology, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran.
Biomed Pharmacother. 2017 Feb;86:441-449. doi: 10.1016/j.biopha.2016.12.049. Epub 2016 Dec 22.
Rosemary, Rosmarinus (R.) officinalis L. is a well-known plant with several useful properties such as analgesic, anti-inflammatory and anti-neurodegenerative. It has been used in folk medicine to alleviate rheumatic pain, stomachache and dysmenorrhea. Rosemary has several constituents such as rosmarinic acid which can be responsible for therapeutic properties been noted with rosemary. The aim of this study was to investigate the potential anti-inflammatory effects of R. officinalis and rosmarinic acid in a rat model of sciatic nerve chronic constriction injury (CCI)-induced neuropathic pain to verify usage of rosemary in folk medicine.
Rats underwent CCI, were treated with either normal saline, ethanolic extract of aerial parts of R. officinalis (400mg/kg, i.p.) or rosmarinic acid (40mg/kg, i.p.) from the day of surgery (day 0) for 14days. The anti-inflammatory effects of R. officinalis extract and rosmarinic acid were evaluated by assessing the levels of some spinal inflammatory markers including cyclooxygenase-2 (COX2), prostaglandin E2 (PGE-2), interleukin 1 beta (IL-1β), matrix metallopeptidase 2 (MMP2) through western blotting and nitric oxide (NO) production via Griess reaction on days 7 and 14 post-surgery.
CCI rats exhibited a marked expression in the levels of inflammatory markers (COX2, PGE-2, IL-1β, MMP2 and NO) on both days 7 (p<0.001) and 14 (p<0.001). Rosmarinic acid and ethanolic extract of R. officinalis were able to decrease amounts of mentioned inflammatory markers on both days 7 (p<0.001) and 14 (p<0.001).
Our data support the traditional use of R. officinalis as an effective remedy for pain relief and inflammatory disorders. It also suggests that the ethanolic extract of R. officinalis and rosmarinic acid through modulating neuro-inflammation might be potential candidates in treating neuropathic pain and different neurological disorders associated with inflammation.
迷迭香（Rosmarinus officinalis L.）是一种广为人知的植物，具有多种有益特性，如镇痛、抗炎和抗神经退行性等。在民间医学中，它被用于缓解风湿痛、胃痛和痛经。迷迭香含有多种成分，如迷迭香酸，这可能是迷迭香具有治疗特性的原因。本研究旨在通过坐骨神经慢性缩窄性损伤（CCI）诱导的神经病理性疼痛大鼠模型，研究罗勒（R. officinalis）和迷迭香酸的潜在抗炎作用，以验证迷迭香在民间医学中的应用。
大鼠接受 CCI 手术，术后第 0 天开始分别给予生理盐水、罗勒地上部分的乙醇提取物（400mg/kg，腹腔注射）或迷迭香酸（40mg/kg，腹腔注射），连续 14 天。通过 Western blot 检测环氧化酶-2（COX2）、前列腺素 E2（PGE-2）、白细胞介素 1β（IL-1β）、基质金属蛋白酶 2（MMP2）等脊髓炎症标志物的水平，以及通过 Griess 反应检测术后第 7 和 14 天的一氧化氮（NO）生成，评估罗勒提取物和迷迭香酸的抗炎作用。
CCI 大鼠在第 7 天（p<0.001）和第 14 天（p<0.001）均表现出炎症标志物（COX2、PGE-2、IL-1β、MMP2 和 NO）水平的显著表达。迷迭香酸和罗勒地上部分的乙醇提取物均能降低第 7 天（p<0.001）和第 14 天（p<0.001）上述炎症标志物的含量。
我们的数据支持罗勒作为一种有效缓解疼痛和炎症性疾病的传统药物的使用。此外，罗勒地上部分的乙醇提取物和迷迭香酸可能通过调节神经炎症，成为治疗神经病理性疼痛和与炎症相关的不同神经疾病的潜在候选药物。
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