Coppola Mariateresa, Arroyo Leonar, van Meijgaarden Krista E, Franken Kees Lmc, Geluk Annemieke, Barrera Luis F, Ottenhoff Tom H M
Dept. of Infectious Diseases, Leiden University Medical Center, PO Box 9600, 2300, RC Leiden, The Netherlands.
Grupo de Inmunología Cellular e Inmunogenética (GICIG), Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.
Tuberculosis (Edinb). 2017 Sep;106:25-32. doi: 10.1016/j.tube.2017.06.001. Epub 2017 Jun 8.
Tuberculosis (TB) occurs in only 3-10% of Mycobacterium tuberculosis (Mtb) infected individuals, suggesting that natural immunity can contain Mtb infection, although this remains poorly understood. Next to T-cells, a potentially protective role for B-cells and antibodies has emerged recently. However, the Mtb antigens involved remain ill-defined. Here, we investigated in a TB-endemic setting IgG levels against 15 Mtb antigens, representing various phases of Mtb infection and known to be potent human T-cell antigens. IgG levels against ESAT6/CFP10, Rv0440, Rv0867c, Rv1737c, Rv2029c, Rv2215, Rv2389c, Rv3616c and Mtb purified protein derivative (PPD) were higher in TB patients than in endemic and non-endemic controls. The only exception was Rv1733c that was preferentially recognized by antibodies from endemic controls compared to TB patients and non-endemic controls, suggesting a potential correlation with control of TB infection and progression. In patients, IgG levels against Ag85B and Rv2029c correlated with Mtb loads, while immunoglobulins against Rv0440 differed between genders. Our results support the potential role of certain Mtb antigen-(Rv1733c) specific antibodies in the control of TB infection and progression, while other Mtb antigen-specific antibodies correlate with TB disease activity and bacillary loads. The findings for Rv1733c agree with previous T-cell results and have implications for including antibody-mediated immunity in designing new strategies to control TB.
结核病（TB）仅在3%-10%的结核分枝杆菌（Mtb）感染个体中发生，这表明自然免疫能够控制Mtb感染，尽管对此仍知之甚少。除了T细胞外，B细胞和抗体最近也显示出潜在的保护作用。然而，所涉及的Mtb抗原仍不明确。在此，我们在结核病流行地区调查了针对15种Mtb抗原的IgG水平，这些抗原代表Mtb感染的不同阶段，并且已知是强效的人类T细胞抗原。结核病患者针对ESAT6/CFP10、Rv0440、Rv0867c、Rv1737c、Rv2029c、Rv2215、Rv2389c、Rv3616c和Mtb纯化蛋白衍生物（PPD）的IgG水平高于流行地区和非流行地区的对照。唯一的例外是Rv1733c，与结核病患者和非流行地区对照相比，流行地区对照的抗体更优先识别该抗原，这表明其与结核病感染和进展的控制可能存在关联。在患者中，针对Ag85B和Rv2029c的IgG水平与Mtb载量相关，而针对Rv0440的免疫球蛋白在不同性别之间存在差异。我们的结果支持某些Mtb抗原（Rv1733c）特异性抗体在结核病感染和进展控制中的潜在作用，而其他Mtb抗原特异性抗体与结核病疾病活动和细菌载量相关。Rv1733c的研究结果与先前的T细胞研究结果一致，并且对于在设计控制结核病的新策略中纳入抗体介导的免疫具有启示意义。
