Kumari Priyanka, Sikri Kriti, Kaur Kohinoor, Gupta Umesh Datta, Tyagi Jaya Sivaswami
Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, India; National JALMA Institute of Leprosy and Other Mycobacterial Diseases, Tajganj, Agra, India.
Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, India.
Tuberculosis (Edinb). 2017 Sep;106:33-37. doi: 10.1016/j.tube.2017.06.003. Epub 2017 Jun 27.
DevR/DosR is a key mediator of 'dormancy' adaptation in Mycobacterium tuberculosis in response to gaseous stresses such as hypoxia that inhibit aerobic mode of respiration. In the present study, a temporal analysis over a 1 year period has revealed robust expression of representative DevR regulon genes devR, hspX and tgs1, during long-term 'dormancy' adaptation to hypoxia. Notably, a predominant proportion of long-term hypoxia-adapted bacteria were characterized by their inability to grow on solid media, accumulation of triacylglycerols and recovery of growth in liquid media. Persistent expression of HspX and the accumulation of triacylglycerols reveal a previously underappreciated role of DevR during adaptation to extended hypoxia, and endorse DevR as an effective target for thwarting the sustained survival of 'dormant' subpopulation of M. tuberculosis.
DevR/DosR是结核分枝杆菌中“休眠”适应的关键调节因子，可响应诸如缺氧等抑制有氧呼吸模式的气体应激。在本研究中，一项为期1年的时间分析显示，在长期“休眠”适应缺氧过程中，代表性的DevR调控子基因devR、hspX和tgs1有强烈表达。值得注意的是，长期适应缺氧的细菌中，很大一部分的特征是无法在固体培养基上生长、三酰甘油积累以及在液体培养基中恢复生长。HspX的持续表达和三酰甘油的积累揭示了DevR在适应长期缺氧过程中一个此前未被充分认识的作用，并支持将DevR作为阻止结核分枝杆菌“休眠”亚群持续存活的有效靶点。
