Postler Thomas S, Pantry Shara N, Desrosiers Ronald C, Ghosh Sankar
Department of Microbiology & Immunology, Columbia University, College of Physicians & Surgeons, New York, NY 10032, USA.
Department of Pathology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
Virology. 2017 Nov;511:30-39. doi: 10.1016/j.virol.2017.08.006. Epub 2017 Aug 10.
Long non-coding RNAs (lncRNAs) are rapidly emerging as important regulators of a diverse array of cellular functions. Here, we describe a meta-analysis of two independent RNA-seq studies to identify lncRNAs that are differentially expressed upon HIV-1 infection. Only three lncRNA genes exhibited altered expression of ≥ 2-fold in HIV-1-infected cells. Of these, the uncharacterized lncRNA LINC00173 was chosen for further study. Both transcript variants of LINC00173 (lnc173 TSV1 and 2) could be detected by qPCR, localized predominantly to the nucleus and were reproducibly up-regulated during infection. Knock-out of the LINC00173 locus did not have detectable effects on HIV-1 replication. Interestingly, however, stimulation of Jurkat T cells with PMA/ionomycin resulted in a decrease of lnc173 expression, and Jurkat cells deficient for lnc173 on average expressed higher levels of specific cytokines than control cells. These data suggest that lnc173 may have a role in the regulation of cytokines in T cells.
长链非编码RNA（lncRNAs）正迅速成为多种细胞功能的重要调节因子。在此，我们描述了一项对两项独立RNA测序研究的荟萃分析，以鉴定在HIV-1感染后差异表达的lncRNAs。在HIV-1感染的细胞中，只有三个lncRNA基因表现出≥2倍的表达改变。其中，未表征的lncRNA LINC00173被选作进一步研究对象。LINC00173的两种转录本变体（lnc173 TSV1和2）均可通过qPCR检测到，主要定位于细胞核，且在感染期间可重复性地上调。敲除LINC00173基因座对HIV-1复制没有可检测到的影响。然而，有趣的是，用佛波酯/离子霉素刺激Jurkat T细胞会导致lnc173表达降低，并且lnc173缺陷的Jurkat细胞平均比对照细胞表达更高水平的特定细胞因子。这些数据表明lnc173可能在T细胞中细胞因子的调节中发挥作用。
