澳洲茄碱逆转人结直肠癌细胞干性和上皮-间充质转化。
Solasodine reverses stemness and epithelial-mesenchymal transition in human colorectal cancer.
机构信息
The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of TCM, Nanjing, China; The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, China; Department of Integrated Traditional and Western Medicine, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of TCM, Nanjing, China.
出版信息
Biochem Biophys Res Commun. 2018 Oct 28;505(2):485-491. doi: 10.1016/j.bbrc.2018.09.094. Epub 2018 Sep 26.
Adverse side effects of conventional chemotherapy, acquired resistance and fatal tumor metastasis of human colorectal cancer (CRC) are propelling the exploration for novel selective anticarcinogens. Solasodine is a main active component isolated from Solanum incanum L that exhibited a potent stemness and invasion inhibitory effect on human colorectal cancer HCT116 cells. Colony Spheroid formation assay showed that solasodine dose-dependently prohibited HCT116 cell stemness. CD133, CD44, Nanog, Oct-4 and Sox-2 were inhibited by solasodine to reverse stemness and similar mechanism was stimulated in vivo. Transwell and scratch wound assays revealed that solasodine impeded HCT116 cell invasion and migration potential strengthened by TGF-β1. Moreover, solasodine attenuated TGF-β1-induced EMT and decreased MMPs while in vivo study showed the same trend. The results of this study implied that solasodine may be a novel therapeutic drug for CRC treatment.
常规化疗的副作用、人结直肠癌（CRC）的获得性耐药和致命肿瘤转移正在推动对新型选择性抗癌药物的探索。茄碱是从茄属植物中分离得到的主要活性成分，对人结直肠癌细胞 HCT116 表现出强大的干性和侵袭抑制作用。集落球形成实验表明，茄碱剂量依赖性地抑制 HCT116 细胞干性。CD133、CD44、Nanog、Oct-4 和 Sox-2 被茄碱抑制，逆转干性，体内也有类似的机制。Transwell 和划痕伤口实验表明，茄碱抑制了 TGF-β1 增强的 HCT116 细胞侵袭和迁移能力。此外，茄碱减弱了 TGF-β1 诱导的 EMT 并降低了 MMPs，而体内研究也显示出相同的趋势。这项研究的结果表明，茄碱可能是治疗 CRC 的一种新型治疗药物。
Zotero 插件