Division of Laboratory Medicine, Department of Pathology, University of Virginia School of Medicine and Health Sciences Center, Charlottesville, VA, United States.
Division of Laboratory Medicine, Department of Pathology, University of Virginia School of Medicine and Health Sciences Center, Charlottesville, VA, United States.
Clin Chim Acta. 2017 Oct;473:9-13. doi: 10.1016/j.cca.2017.08.008. Epub 2017 Aug 10.
Transport of blood samples through pneumatic tube systems (PTSs) generates air bubbles in transported blood samples and, with increasing duration of transport, the appearance of hemolysis. We investigated the role of air-bubble formation in PTS-induced hemolysis.
Air was introduced into blood samples for 0, 1, 3 or 5min to form air bubbles. Hemolysis in the blood was assessed by (H)-index, lactate dehydrogenase (LD) and potassium in plasma. In an effort to prevent PTS-induced hemolysis, blood sample tubes were completely filled, to prevent air bubble formation, and compared with partially filled samples after PTS transport. We also compared hemolysis in anticoagulated vs clotted blood subjected to PTS transport.
As with transport through PTSs, the duration of air bubble formation in blood by a gentle stream of air predicted the extent of hemolysis as measured by H-index (p<0.01), LD (p<0.01), and potassium (p<0.02) in plasma. Removing air space in a blood sample prevented bubble formation and fully protected the blood from PTS-induced hemolysis (p<0.02 vs conventionally filled collection tube). Clotted blood developed less foaming during PTS transport and was partially protected from hemolysis vs anticoagulated blood as indicated by lower LD (p<0.03) in serum than in plasma after PTS sample transport.
Prevention of air bubble formation in blood samples during PTS transport protects samples from hemolysis.
通过气动输送管系统（PTS）运输血样会在运输的血样中产生气泡，并且随着运输时间的延长，会出现溶血现象。我们研究了气泡形成在 PTS 诱导溶血中的作用。
将空气引入血样中 0、1、3 或 5 分钟以形成气泡。通过 H 指数、乳酸脱氢酶（LD）和血浆中的钾来评估血液中的溶血情况。为了防止 PTS 诱导的溶血，将血样管完全装满，以防止气泡形成，并与 PTS 运输后的部分填充样品进行比较。我们还比较了抗凝和凝块血在 PTS 运输后的溶血情况。
与 PTS 运输一样，通过轻轻的空气流在血液中形成气泡的时间预测了溶血的程度，如 H 指数（p<0.01）、LD（p<0.01）和血浆中的钾（p<0.02）所示。在血样中去除气隙空间可防止气泡形成，并完全防止 PTS 诱导的溶血（与常规填充采集管相比，p<0.02）。凝块血在 PTS 运输过程中产生的泡沫较少，与抗凝血相比，溶血程度部分得到保护，如 PTS 样品运输后血清中的 LD （p<0.03）低于血浆中的 LD 所示。
在 PTS 运输过程中防止血样中气泡的形成可保护血样免受溶血。
