血管内皮生长因子（VEGF）基因多态性（rs699947、rs833061和rs2010963）与南印度泰米尔人的银屑病风险
Vascular endothelial growth factor (VEGF) gene polymorphisms (rs699947, rs833061, and rs2010963) and psoriatic risk in South Indian Tamils.
作者信息
Sudhesan Anjana, Rajappa Medha, Chandrashekar Laxmisha, Ananthanarayanan P H, Thappa D M, Satheesh Santhosh, Chandrasekaran Adithan
机构信息
Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
出版信息
Hum Immunol. 2017 Oct;78(10):657-663. doi: 10.1016/j.humimm.2017.08.004. Epub 2017 Aug 10.
BACKGROUND
Psoriasis is a chronic inflammatory disease of the skin. Vascular endothelial growth factor (VEGF), a pro-angiogenic factor, is involved in the pathogenesis of psoriasis. Being highly polymorphic, several SNPs of VEGF have been reported to be associated with increased risk of psoriasis.
OBJECTIVES
We determined the association of VEGF gene polymorphisms with risk of psoriasis in South Indian Tamils.
METHODS
300 cases of psoriasis and 300 controls were recruited in this case-control study. Genotyping of SNPs of VEGF gene was done using Taqman 5' allele discrimination assay. Estimation of VEGF levels in plasma was done by ELISA.
RESULTS
VEGF (rs2010963) polymorphism and the CTC haplotype were found to confer an increased risk of psoriasis. However, two other VEGF SNPs, rs833061, and rs699947, showed no association with psoriasis susceptibility. VEGF levels were higher in patients with psoriasis, as compared with controls and significantly correlated with disease severity.
CONCLUSIONS
Our results indicate that VEGF (rs2010963) polymorphism and CTC haplotype of the VEGF SNPs (rs699947, rs833061, and rs2010963) confer an increased risk of psoriasis in the South Indian Tamil population. Plasma VEGF levels are higher in patients with psoriasis, as compared with controls and are significantly correlated with disease severity.
背景
银屑病是一种慢性皮肤炎症性疾病。血管内皮生长因子（VEGF）是一种促血管生成因子，参与银屑病的发病机制。VEGF具有高度多态性，据报道其多个单核苷酸多态性（SNP）与银屑病风险增加有关。
目的
我们确定了VEGF基因多态性与南印度泰米尔人患银屑病风险之间的关联。
方法
在这项病例对照研究中招募了300例银屑病患者和300名对照。使用Taqman 5'等位基因鉴别分析对VEGF基因的SNP进行基因分型。通过酶联免疫吸附测定（ELISA）法测定血浆中VEGF水平。
结果
发现VEGF（rs2010963）多态性和CTC单倍型会增加患银屑病的风险。然而，另外两个VEGF SNP，即rs833061和rs699947，与银屑病易感性无关。与对照组相比，银屑病患者的VEGF水平更高，且与疾病严重程度显著相关。
结论
我们的结果表明，VEGF（rs2010963）多态性以及VEGF SNPs（rs699947、rs833061和rs2010963）的CTC单倍型会增加南印度泰米尔人群患银屑病的风险。与对照组相比，银屑病患者的血浆VEGF水平更高，且与疾病严重程度显著相关。
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