Tripodi Armando, Chantarangkul Veena
Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Department of Clinical Sciences and Community Health, Università degli Studi di Milano and IRCCS Cà Granda Maggiore Hospital Foundation, via pace 9, 20122, Milan, Italy.
Methods Mol Biol. 2017;1646:177-183. doi: 10.1007/978-1-4939-7196-1_15.
Actvated partial thromboplastin time and its congener test Silica clotting time are used for the laboratory detection of lupus anticoagulants. Their performance consists of mixing test plasma with activators of the contact coagulation factors, phospholipids and calcium chloride, and recording the clotting time. Here we describe the principle, performance and extensive practical details of the two methods, the problems that may be encountered and how they can be identified and overcome.
活化部分凝血活酶时间及其同类检测——硅凝血时间用于实验室检测狼疮抗凝物。其操作是将测试血浆与接触凝血因子激活剂、磷脂和氯化钙混合，然后记录凝血时间。在此，我们描述这两种方法的原理、操作及大量实际细节、可能遇到的问题以及如何识别和克服这些问题。
