全血中血小板功能评估的流式细胞术方案

Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood.

作者信息

Pasalic Leonardo, Pennings Gabrielle J, Connor David, Campbell Heather, Kritharides Leonard, Chen Vivien M

机构信息

Departments of Clinical and Laboratory Haematology, Institute of Clinical Pathology and Medical Research, WestmeadHospital, Westmead, NSW 2145, Australia.

Sydney Centres for Thrombosis and Haemostasis, Westmead, NSW, Australia.

出版信息

Methods Mol Biol. 2017;1646:369-389. doi: 10.1007/978-1-4939-7196-1_28.

DOI:10.1007/978-1-4939-7196-1_28

PMID:28804842

Abstract

Flow cytometry is a powerful tool for rapid evaluation of multiple functional properties of large numbers of platelets in whole blood. In the following chapter, we provide a number of flow cytometry-based protocols broadly aimed at (1) assessment of constitutively expressed platelet membrane receptors to diagnose inherited platelet bleeding disorders and (2) investigation of basal and agonist-induced platelet functional responses including generation of platelet-leukocyte aggregates, alpha and dense granule release, calcium flux, and phosphatidylserine exposure.

摘要

流式细胞术是一种用于快速评估全血中大量血小板多种功能特性的强大工具。在接下来的章节中，我们提供了一些基于流式细胞术的方案，其主要目的广泛包括：（1）评估组成性表达的血小板膜受体，以诊断遗传性血小板出血性疾病；（2）研究基础和激动剂诱导的血小板功能反应，包括血小板 - 白细胞聚集体的生成、α和致密颗粒释放、钙通量以及磷脂酰丝氨酸暴露。