Nanobiofar Group, Center for Research in Molecular Medicine & Chronic Diseases (CIMUS), Universidade de Santiago de Compostela, 15706 Santiago de Compostela, Spain.
ZF-Screens B.V., 2333 Leiden, The Netherlands.
Nanomedicine (Lond). 2017 Sep;12(17):2069-2082. doi: 10.2217/nnm-2017-0078. Epub 2017 Aug 14.
To assess the capacity of a new drug delivery nanocapsule (NC) with a double shell of hyaluronic acid and protamine to overcome biological barriers using the zebrafish model.
MATERIALS & METHODS: NCs were prepared by the solvent displacement method, tagged with fluorescent makers and physicochemically characterized. Toxicity was evaluated according to the Fish Embryo Acute Toxicity test, and permeability was tested by exposing zebrafish, with and without chorion, to the fluorescent NCs.
Toxicity of NCs was very low as compared with that of a control nanoemulsion. Double-shell NCs were able to cross chorion and skin.
Beyond the potential value of hyaluronic acid:protamine NCs for overcoming epithelial barriers, this works highlights the utility of zebrafish for fast screening of nanocarriers.
使用斑马鱼模型评估具有双透明质酸和鱼精蛋白壳的新型药物递送纳米囊（NC）克服生物屏障的能力。
通过溶剂置换法制备 NC，标记荧光标记物并进行物理化学表征。根据鱼类胚胎急性毒性试验评估毒性，通过将带有和不带有卵壳的斑马鱼暴露于荧光 NC 来测试渗透性。
与对照纳米乳液相比，NC 的毒性非常低。双壳 NC 能够穿过卵壳和皮肤。
除了透明质酸：鱼精蛋白 NC 克服上皮屏障的潜在价值外，这项工作还强调了斑马鱼在快速筛选纳米载体方面的实用性。
