Maharaj R, Robb F T, Woods D R
Arch Microbiol. 1986 Oct;146(1):30-4. doi: 10.1007/BF00690154.
Glutamine synthetase (GS) synthesis in Vibrio alginolyticus was regulated by temperature, oxygen and nitrogen levels. A GS gene, glnA from V. alginolyticus was cloned on a 5.67 kb insert in the recombinant plasmid pRM210, which enabled Escherichia coli glnA, ntrB, ntrC deletion mutants to utilize (NH4)2SO4 as a sole source of nitrogen. The V. alginolyticus glnA gene was expressed from a regulatory region contained within the cloned fragment. V. alginolyticus glnA expression from pRM210 was subject to regulation by temperature, oxygen and nitrogen levels. GS specific activity in an E. coli wild-type strain was not affected by temperature or oxygen. pRM211 was a deletion derivative of pRM210 and GS production by pRM211 was not regulated by temperature, oxygen or nitrogen levels in E. coli.
溶藻弧菌中谷氨酰胺合成酶（GS）的合成受温度、氧气和氮水平的调节。从溶藻弧菌中克隆出一个GS基因glnA，它位于重组质粒pRM210的一个5.67 kb插入片段上，该质粒能使大肠杆菌glnA、ntrB、ntrC缺失突变体利用硫酸铵作为唯一氮源。溶藻弧菌glnA基因从克隆片段内包含的调控区域表达。pRM210中溶藻弧菌glnA的表达受温度、氧气和氮水平的调节。大肠杆菌野生型菌株中的GS比活性不受温度或氧气的影响。pRM211是pRM210的缺失衍生物，pRM211在大肠杆菌中产生的GS不受温度、氧气或氮水平的调节。
